Unexpected findings from target analysis of immunoglobulin E and its receptor

The membrane receptor for immunoglobulin E (IgE) and its ligand, IgE, were irradiated with high-energy electrons. Loss of binding activity was measured for each, and the size of the functional targets was assessed. In both cases, the target size was substantially smaller than the covalent structure...

Full description

Saved in:
Bibliographic Details
Published in:Biochemistry (Easton) 1981-11, Vol.20 (23), p.6589-6594
Main Authors: Fewtrell, Clare, Kempner, Ellis, Poy, George, Metzger, Henry
Format: Article
Language:English
Subjects:
Acetylglucosaminidase - metabolism
alpha radiation
Animals
Basophils - immunology
Cell Membrane - immunology
Glucuronidase - metabolism
immunoglobulin E
Immunoglobulin E - metabolism
Kinetics
Leukemia, Experimental - enzymology
Leukemia, Experimental - immunology
Molecular Weight
Rats
Receptors, IgE
Receptors, Immunologic - metabolism
Receptors, Immunologic - radiation effects
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The membrane receptor for immunoglobulin E (IgE) and its ligand, IgE, were irradiated with high-energy electrons. Loss of binding activity was measured for each, and the size of the functional targets was assessed. In both cases, the target size was substantially smaller than the covalent structure of the molecule. The direction of this discrepancy is unprecedented on the basis of experience with loss of enzymatic activity by irradiation; indeed, two enzymes which were present in the receptor preparations gave expected values when measured simultaneously. We suggest that in instances where a function such as ligand binding resides in a conformationally stable domain, radiation inactivation may be capable of revealing this.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00526a011