Formation of matrix vesicles by cultured chick embryo chondrocytes

This report shows that when chondrocytes from the chick embryo tibiotarsus are cultured in monolayers, they form vesicles which are released into the culture medium. These vesicles possess all of the properties of matrix vesicles which have been isolated from cartilagenous tissues. They contain high...

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Bibliographic Details
Published in:The Journal of biological chemistry 1981-12, Vol.256 (23), p.12607-12611
Main Authors: Glaser, J H, Conrad, H E
Format: Article
Language:English
Subjects:
Animals
Calcium - analysis
Cartilage - embryology
Cartilage - metabolism
Cartilage - ultrastructure
Cell Fractionation
Cell Membrane - ultrastructure
Cells, Cultured
Chick Embryo
chick embryos
chondrocytes
Microscopy, Electron
vesicles
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Summary:This report shows that when chondrocytes from the chick embryo tibiotarsus are cultured in monolayers, they form vesicles which are released into the culture medium. These vesicles possess all of the properties of matrix vesicles which have been isolated from cartilagenous tissues. They contain high levels of alkaline phosphatase and Ca super(2+), and they have phospholipid and cholesterol contents very similar to those reported for matrix vesicles. The results suggest that the chondrocyte monolayer system may serve as a model for studying the events leading to the formation and calcification of matrix vesicles by chondrocytes.
ISSN:0021-9258
DOI:10.1016/S0021-9258(18)43318-2