Assembly of functional b-type cytochromes in membranes from a 5-aminolaevulinic acid-requiring mutant of Escherichia coli

It is the aim of this study to investigate which cytochromes are reconstituted during incubation of haemdeficient membranes with haematin and ATP. The reconstituted cytochromes have been studied by potentiometric redox titrations and low-temperature spectrophotometry of membrane preparations, and co...

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Bibliographic Details
Published in:FEBS letters 1981-08, Vol.131 (2), p.346-350
Main Authors: Reid, Graeme A., Haddock, Bruce A., Ingledew, W.John
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Aminolevulinic Acid - metabolism
ATP
Cell Membrane - metabolism
Cytochrome b Group
cytochromes
Cytochromes - metabolism
Escherichia coli
Escherichia coli - genetics
Escherichia coli - metabolism
Heme - analogs & derivatives
Heme - deficiency
Hemin - metabolism
Levulinic Acids - metabolism
membranes
Multienzyme Complexes - metabolism
NADH, NADPH Oxidoreductases - metabolism
Oxidation-Reduction
reconstitution
Spectrophotometry
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Summary:It is the aim of this study to investigate which cytochromes are reconstituted during incubation of haemdeficient membranes with haematin and ATP. The reconstituted cytochromes have been studied by potentiometric redox titrations and low-temperature spectrophotometry of membrane preparations, and compared with the cytochromes found in prototrophic E. coli . The requirement for ATP during reconstitution has been further investigated by similar studies with haem-deficient membranes which had been incubated with haematin in the absence of ATP; cytochromes are apparently reconstituted but these membranes lack NADH oxidase activity.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(81)80400-0