A technique for the immunohistochemical localization of prostaglandin E

Prostaglandin E (PGE) has been localized via the unlabeled antibody technique in freeze-dried and ethanol-fixed cryostat sections. Discrete perivascular and stromal localization was present in the uterus prepared by the method presented, but not in classically fixed specimens. Absorption of the anti...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 1977-12, Vol.25 (12), p.1291-1296
Main Authors: Van Orden, DE, Klemm, JM, Redick, JA
Format: Article
Language:English
Subjects:
Animals
Female
Fixatives
Histological Techniques
Immunoenzyme Techniques
Prostaglandins E - analysis
Prostaglandins E - immunology
Rats
Uterus - cytology
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Description
Summary:Prostaglandin E (PGE) has been localized via the unlabeled antibody technique in freeze-dried and ethanol-fixed cryostat sections. Discrete perivascular and stromal localization was present in the uterus prepared by the method presented, but not in classically fixed specimens. Absorption of the anti-PGE by addition of free PGE was ineffective; whereas, removal of PGE-reactive antibodies from the anti-serum was effectively accomplished with an Affigel-101-PGE immunoadsorbant column.
ISSN:0022-1554
1551-5044
DOI:10.1177/25.12.336783