Antibodies to left-handed Z-DNA bind to interband regions of Drosophila polytene chromosomes

Antibodies which are specific to the Z–DNA conformation have been purified and characterized on the basis of their binding to three different DNA polymers which can form this left-handed helix. These antibodies bind specifically to polytene chromosomes of Drosophila melanogaster as visualized by flu...

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Bibliographic Details
Published in:Nature (London) 1981-12, Vol.294 (5840), p.417-422
Main Authors: Nordheim, A, Pardue, M.L, Lafer, E.M, Moller, A, Stollar, B.D, Rich, A
Format: Article
Language:English
Subjects:
animal breeding
animal genetics
Animals
arthropods
Base Sequence
Chromosome Banding
Chromosomes - ultrastructure
DNA - immunology
Drosophila melanogaster - genetics
entomology
Fluorescent Antibody Technique
Gene Expression Regulation
Humanities and Social Sciences
multidisciplinary
Nucleic Acid Conformation
Science
Science (multidisciplinary)
Structure-Activity Relationship
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Summary:Antibodies which are specific to the Z–DNA conformation have been purified and characterized on the basis of their binding to three different DNA polymers which can form this left-handed helix. These antibodies bind specifically to polytene chromosomes of Drosophila melanogaster as visualized by fluorescent staining. The staining is found in the interband regions and its intensity varies among different interbands in a reproducible manner. This is the first identification of the Z-DNA conformation in material of biological origin.
ISSN:0028-0836
1476-4687
DOI:10.1038/294417a0