In vitrocleavage of 45S pre-ribosomal RNA and of giant heterogeneous RNA extracted from human leukemic cells

45S ribosomal precursor RNA and large heterogeneous RNA molecules (greater than 45S) extracted from human leukemic cells were incubated in vitro with purified RNase III, which specifically attacks double-helical RNA regions. About 50% of the ribosomal precursor was cleaved into two major fragments s...

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Bibliographic Details
Published in:Molecular biology reports 1977-12, Vol.3 (6), p.403-411
Main Authors: Torelli, U L, Ferrari, S, Torelli, G M, Cadossi, R, Montagnani, G, Narni, F
Format: Article
Language:English
Subjects:
Cells, Cultured
Centrifugation, Density Gradient
Humans
Leukemia - analysis
Ribonucleases - metabolism
RNA - analysis
RNA, Ribosomal - analysis
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Summary:45S ribosomal precursor RNA and large heterogeneous RNA molecules (greater than 45S) extracted from human leukemic cells were incubated in vitro with purified RNase III, which specifically attacks double-helical RNA regions. About 50% of the ribosomal precursor was cleaved into two major fragments sedimenting at 28S and 32S respectively. A limited number of cleavages was also introduced in about 40% of heterogeneous RNA molecules sedimenting faster than 45S, causing a partial 'shift' to a polydisperse distribution in the 10S-45S range.
ISSN:0301-4851