Rat Hepatocyte Primary Cell Cultures. III. Improved Dissociation and Attachment Techniques and the Enhancement of Survival by Culture Medium

The conditions for obtaining representative, adult rat hepatocyte primary cell cultures were improved such that viable yields of 50% of the liver were produced which gave rise to cultures representing 30% of the liver. The survival of these cultures in various media was compared revealing that in co...

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Bibliographic Details
Published in:In Vitro 1977-12, Vol.13 (12), p.809-817
Main Authors: Williams, Gary M., Bermudez, Edilberto, Dominick Scaramuzzino
Format: Article
Language:English
Subjects:
Animals
Cell Adhesion
Cell Count
Cell culture techniques
Cell Separation - methods
Cell Survival - drug effects
Cells, Cultured
Culture Media
Cultured cells
Epithelial cells
Flasks
Galactose - pharmacology
HeLa cells
Hepatocytes
In Vitro Techniques
Liver
Liver cells
Perfusion
Rats
Viability
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Summary:The conditions for obtaining representative, adult rat hepatocyte primary cell cultures were improved such that viable yields of 50% of the liver were produced which gave rise to cultures representing 30% of the liver. The survival of these cultures in various media was compared revealing that in complex media, particularly containing galactose, survival was improved.
ISSN:0073-5655
1475-2689
DOI:10.1007/BF02615128