Purification and amino acid sequence of mating factor from Saccharomyces cerevisiae [yeasts]

Mating factor is a peptide excreted into the culture fluid by α-mating type cells of Saccharomyces cerevisiae X-2180 1B. The purification of the mating factor was carried out by ion exchange chromatography on phosphocellulose and Amberlite IRC 50 columns, followed by gel filtration on a Sephadex LH...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1977-12, Vol.82 (6), p.1681-1687
Main Authors: TANAKA, Takaharu, KITA, Hiroshi, MURAKAMI, Taeko, NARITA, Kozo
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Peptide Fragments - analysis
Peptides - isolation & purification
Peptides - physiology
Saccharomyces cerevisiae - physiology
Spectrophotometry, Ultraviolet
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Summary:Mating factor is a peptide excreted into the culture fluid by α-mating type cells of Saccharomyces cerevisiae X-2180 1B. The purification of the mating factor was carried out by ion exchange chromatography on phosphocellulose and Amberlite IRC 50 columns, followed by gel filtration on a Sephadex LH 20 column. The factor thus prepared was a peptide composed of Lys1, His1 Trp2 Gln2 Pro2 Gly1 Met1 Leu2 and Tyr1 and was able to induce morphological changes on a-mating type cells at a concentration of 5 pg/ml. The amino acid sequence of the mating factor was determined by the manual Edman degradation method using intact mating factor and its thermolytic peptides. The C-terminalamino acid residue was determined by digesting the factor with carboxypeptidase A. The complete amino acid sequence of the mating factor was established to be as follows: Trp- His- Trp- Leu- Gln- Leu- Lys- Pro- Gly- Gln- Pro- Met- Tyr.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a131864