Structural and immunological studies of the Haemophilus influenzae type d capsular polysaccharide

Employing a combination of chemical and spectroscopic techniques, the structure of the Haemophilus influenzae type d capsular polysaccharide was found to be →4)-β- d-GlcNAc-(1→3)-β- d-ManNAcA-(1→. l-Alanine, l-serine, and l-threonine, in the molar ratios of ∼1.0:1.0:0.3, were linked to C-6 of the d-...

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Bibliographic Details
Published in:Carbohydrate research 1981-11, Vol.97 (2), p.293-306
Main Authors: Tsui, Fai-Po, Schneerson, Rachel, Boykins, Robert A., Karpas, Arthur B., Egan, William
Format: Article
Language:English
Subjects:
Carbohydrate Conformation
Carbohydrate Sequence
Haemophilus influenzae - immunology
Immunodiffusion
Magnetic Resonance Spectroscopy
Polysaccharides, Bacterial - immunology
Precipitin Tests
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Summary:Employing a combination of chemical and spectroscopic techniques, the structure of the Haemophilus influenzae type d capsular polysaccharide was found to be →4)-β- d-GlcNAc-(1→3)-β- d-ManNAcA-(1→. l-Alanine, l-serine, and l-threonine, in the molar ratios of ∼1.0:1.0:0.3, were linked to C-6 of the d-mannosyluronic residue as amides; the (serine + alanine + threonine) to ManNAcA ratio was ∼0.95:1.0. Removal of the amino acids by mild hydrolysis with sodium hydroxide resulted in a material that was cross-reactive with the native, type d polymer. The base-treated, type d polysaccharide was not observed to cross-react with either the H. influenzae type e or Escherichia coli K7 capsular polysaccharide, both of which are structurally similar to type d.
ISSN:0008-6215
1873-426X
DOI:10.1016/S0008-6215(00)80675-8