Deciliation: A stressful event for Paracentrotus lividus embryos

In this report, by using mono- and two-dimensional electrophoretic analysis, we demonstrate that deciliation on sea urchin embryos induces a stress response. Deciliation indeed causes not only the activation of ciliary subroutine, but also a transient decrease of bulk protein synthesis. This decreas...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1998-07, Vol.248 (3), p.628-634
Main Authors: Casano, C, Roccheri, M C, Onorato, K, Cascino, D, Gianguzza, F
Format: Article
Language:English
Subjects:
Animals
Cell Nucleus - physiology
Cell Nucleus - ultrastructure
Cilia - physiology
Cytoplasm - physiology
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Embryo, Nonmammalian - physiology
Embryo, Nonmammalian - ultrastructure
Gastrula - physiology
Gastrula - ultrastructure
Marine
Methionine - metabolism
Paracentrotus lividus
Protein Biosynthesis
Proteins - isolation & purification
Regeneration
Saline Solution, Hypertonic
Sea Urchins - embryology
Stress, Physiological
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Summary:In this report, by using mono- and two-dimensional electrophoretic analysis, we demonstrate that deciliation on sea urchin embryos induces a stress response. Deciliation indeed causes not only the activation of ciliary subroutine, but also a transient decrease of bulk protein synthesis. This decrease is in agreement with our previous results on heat shock response in sea urchin, although deciliation does not induce the expression of the same main hsp set. We were able to characterize one main deciliation-stress protein of 40 kDa whose expression is transiently induced by deciliation and whose localisation is likely to be nuclear.
ISSN:0006-291X
DOI:10.1006/bbrc.1998.9032