Sequencing of short RNA oligomers by proton nuclear magnetic resonance

Here, the author present a non-destructive RNA sequencing technique for short oligoribonunleotides which is independent methodologically from the existing chemical and enzymatic procedures. A spectroscopic procedure, proton nuclear magnetic resonance ( super(1)H NMR) is used which requires a pure sa...

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Bibliographic Details
Published in:FEBS letters 1981-12, Vol.136 (1), p.65-69
Main Authors: Hader, P.A., Neilson, T., Alkema, D., Kofoid, E.C., Ganoza, M.C.
Format: Article
Language:English
Subjects:
Base Sequence
Magnetic Resonance Spectroscopy
Oligonucleotides
Oligoribonucleotides
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Summary:Here, the author present a non-destructive RNA sequencing technique for short oligoribonunleotides which is independent methodologically from the existing chemical and enzymatic procedures. A spectroscopic procedure, proton nuclear magnetic resonance ( super(1)H NMR) is used which requires a pure sample (500-800 mu g) of the RNA fragment to be sequenced. The procedure is based on the fact that the resonances of the non-exchangeable aromatic and anomeric protons of adenosine, guanosine, cytidine and uridine exhibit changes in their particular chemical shifts which depend on the bases immediately surrounding a particular residue in a given sequence. These sequence effects on chemical shifts of protons on a particular residue do not extend significantly beyond the next-nearest neighbour at 70 degree C.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(81)81214-8