Elimination of Mycoplasma hyorhinis Infections from Four Cell Lines

Four monolayer mammalian cell lines were cured of Mycoplasma hyorhinis infections by cloning in microtiter dishes in the presence of tetracycline and kanamycin. During cloning, cultures were refed with fresh antibiotic containing medium every 2 or 3 d for 14 d and were then cultured without effectiv...

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Bibliographic Details
Published in:In vitro 1981-11, Vol.17 (11), p.993-996
Main Authors: Gurney, Theodore, Margaret J. Woolf, Leslie J. Abplanalp, Nichole H. Mc Kittrick, Jeanne N. Dietz, Barry C. Cole
Format: Article
Language:English
Subjects:
Animals
Antibiotics
Cell culture techniques
Cell Line
Cell lines
Clone Cells - microbiology
Cultured cells
Fluorescence
HeLa cells
Humans
Infections
Kanamycin - pharmacology
L cells
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Mycoplasma
Mycoplasma - growth & development
Mycoplasma hyorhinis
Tetracycline - pharmacology
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Summary:Four monolayer mammalian cell lines were cured of Mycoplasma hyorhinis infections by cloning in microtiter dishes in the presence of tetracycline and kanamycin. During cloning, cultures were refed with fresh antibiotic containing medium every 2 or 3 d for 14 d and were then cultured without effective antibiotics for at least 21 d. From the four lines we recovered 29 clones, none of which were infected after treatment as judged by the lack of extranuclear fluorescence after staining with the fluorochrome Hoechst 33258, and by normal autoradiographic labeling of the cells by tritiated nucleosides. One clone from each line was tested further by attempted culture of mycoplasmas and was also judged to be uninfected. Infection has not reappeared in any of the clones after extensive culture in the absence of the effective antibiotics.
ISSN:0073-5655
DOI:10.1007/BF02618424