Sterically stabilized phospholipids attenuate human neutrophils chemotaxis in vitro

• Published in: Life sciences (1973) 1998, Vol.63 (8), p.693-699
• Main Authors: Hatipoglu, Umur, Gao, Xiao-pei, Verral, Stephen, Séjourné, Florence, Pitrak, David, Alkan-Önyüksel, Hayat, Rubinstein, Israel
• Format: Article
• Language: English
• Subjects: Chemotaxis, Leukocyte - drug effects; Complement System Proteins; Drug Stability; Humans; inflammation; leukocytes; Liposomes; N-Formylmethionine Leucyl-Phenylalanine - pharmacology; Neutrophils - drug effects; Neutrophils - physiology; Opsonin Proteins; PEG-DSPE; Phosphatidylethanolamines - pharmacology; Phospholipids - administration & dosage; Phospholipids - pharmacology; Polyethylene Glycols - pharmacology; vasoactive intestinal peptide; Vasoactive Intestinal Peptide - administration & dosage; Zymosan - pharmacology
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/S0024-3205(98)00320-8

The purpose of this study was to determine whether sterically stabilized liposomes (SSL) and poly(ethylene glycol)-distearoylphosphatidylethanolamine (PEG-DSPE) attenuate polymorphonuclear neutrophils (PMNs) chemotaxis in vitro and, if so, whether incorporation of vasoactive intestinal peptide (VIP), a pleiotropic neuropeptide, on the surface of SSL amplifies SSL-induced responses. Using a modified blind-well chamber chemotaxis assay, we found that N-formyl-methionyl-leucyl-phenylalanine (FMLP; 0.1 μM) and zymosan opsonized with purified human complement (2 × 10 9 yeast wall particles/ml) elicit significant human PMNs chemotaxis (95 ± 9 and 103 ± 3 cells/high power field; p < 0.05). These effects are significantly attenuated by SSL and PEG-DSPE (p < 0.05). By contrast, aqueous VIP and VIP on SSL have no significant effects on FMLP- and zymosan-induced responses. We conclude that certain sterically stabilized liposomes and phospholipids attenuate human PMNs chemotaxis in vitro and that VIP does not modulate this response.