Quantification of Crustacean Hyperglycemic Hormone by ELISA in Hemolymph of the Lobster,Homarus americanus,Following Various Stresses

An ELISA was developed for the crustacean hyperglycemic hormone (CHH) from the lobster,Homarus americanus.It is sensitive to as little as 0.2 fmol of peptide. The assay was used to measure CHH in the hemolymph of intact lobsters after various environmental stresses. Increases in CHH were observed fo...

Full description

Saved in:
Bibliographic Details
Published in:General and comparative endocrinology 1998-09, Vol.111 (3), p.359-366
Main Authors: Chang, Ernest S., Keller, Rainer, Chang, Sharon A.
Format: Article
Language:English
Subjects:
Animals
Enzyme-Linked Immunosorbent Assay
Hemolymph - chemistry
Homarus americanus
Hyperglycemia - metabolism
Invertebrate Hormones - analysis
Marine
Nephropidae - metabolism
Neurosecretory Systems - physiology
Sensitivity and Specificity
Stress, Physiological - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An ELISA was developed for the crustacean hyperglycemic hormone (CHH) from the lobster,Homarus americanus.It is sensitive to as little as 0.2 fmol of peptide. The assay was used to measure CHH in the hemolymph of intact lobsters after various environmental stresses. Increases in CHH were observed following emersion, exposure to high temperatures (23° and 28°C), and salinity stress (50 and 150% seawater). During emersion, concentrations of hemolymph glucose increased concomitantly with increases in CHH. Significant levels of hemolymph CHH were also measured in lobsters that had been eyestalk-ablated. These latter observations indicate that there may be a source of CHH other than the X-organ/sinus gland in the lobster.
ISSN:0016-6480
1095-6840
DOI:10.1006/gcen.1998.7120