Influence of biliary obstruction on neutrophil chemotaxis

The effect of obstructive jaundice on neutrophil chemotactic function was investigated, with a potent chemotactic factor, IL-8 (recombinant rat GRO-beta), in rats that received 7-day bile duct ligation. Carrageenin or IL-8 was injected into a preformed air pouch, and exudate was collected 4 h later...

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Bibliographic Details
Published in:Journal of gastroenterology 1998-08, Vol.33 (4), p.536-540
Main Authors: Yanagitani, K, Kubota, Y, Tsuji, K, Yamamoto, S, Amoh, Y, Takaoka, M, Ogura, M, Inoue, K
Format: Article
Language:English
Subjects:
Animals
Carrageenan - metabolism
Chemotaxis, Leukocyte
Cholestasis - enzymology
Cholestasis - metabolism
Interleukin-8 - metabolism
Male
Neutrophils - metabolism
Peroxidase - metabolism
Rats
Rats, Wistar
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Summary:The effect of obstructive jaundice on neutrophil chemotactic function was investigated, with a potent chemotactic factor, IL-8 (recombinant rat GRO-beta), in rats that received 7-day bile duct ligation. Carrageenin or IL-8 was injected into a preformed air pouch, and exudate was collected 4 h later for measurement of myeloperoxidase activity. In vitro chemotaxis of peripheral neutrophils to IL-8 was evaluated by a modified Boyden chamber method. Both carrageenin and IL-8 induced significantly pronounced intra-air pouch neutrophil recruitment in the bile duct-ligated group compared with a sham-ligated group. In vitro neutrophil chemotaxis was significantly increased in the bile duct-ligated group compared with the sham-ligated group. The present experimental model suggests enhanced neutrophil chemotaxis to IL-8 in obstructive jaundice.
ISSN:0944-1174
1435-5922
DOI:10.1007/s005350050128