Determination of Flurbiprofen and Ibuprofen in Dog Serum with Automated Sample Preparation

• Published in: Journal of pharmaceutical sciences 1981-12, Vol.70 (12), p.1347-1349
• Main Authors: Snider, B.G., Beaubien, L.J., Sears, D.J., Rahn, P.D.
• Format: Article
• Language: English
• Subjects: Animals; Autoanalysis - methods; Bioavailability-determination of flurbiprofen and ibuprofen in dog serum with automated sample preparation; Chromatography, High Pressure Liquid - methods; Dogs; Flurbiprofen - blood; Flurbiprofen-determination in dog serum with automated sample preparation; Hydrogen-Ion Concentration; Ibuprofen - blood; Ibuprofen-determination in dog serum with automated sample preparation; Propionates - blood
• ISSN: 0022-3549; 1520-6017
• DOI: 10.1002/jps.2600701215

for the determination of flurbiprofen and ibuprofen in dog serum were developed using high‐performance liquid chromatography and automated serum extraction. Sample extraction was automated by use of cartridges packed with a styrene‐divinylbenzene macroreticular resin in a microprocessor‐controlled centrifugal system. The average recoveries were 98.9% for flurbiprofen and 94.5% for ibuprofen. The limits of detection were ˜0.04 μg/ml for flurbiprofen at 254 nm and 0.5 μg/ml for ibuprofen at 230 nm. The relative standard deviations for the determination of a laboratory standard between days was 2.4% (20 μg/ml) for flurbiprofen and 1.7% (13 μg/ml) for ibuprofen. Peak height ratios were linear with concentrations of 0.04‐100 μg/ml for flurbiprofen and 1.0‐50 μg/ml for ibuprofen. These methods are simple, rapid, sensitive, and specific. The use of an automated sample preparation procedure improved the between‐day precision by a factor of two when compared to a manual extraction procedure. These methods were applied to bioavailability studies in dogs.