Identification of an additional isoform of STAT5 expressed in immature macrophages

We are interested in understanding the molecular basis of macrophage (Mphi) differentiation and activation by cytokines. Recent reports have suggested that the transcription factor STAT5 may play a role in Mphi differentiation. In the experiments described here, we assessed the expression of STAT5-r...

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Bibliographic Details
Published in:The Journal of immunology (1950) 1998-08, Vol.161 (4), p.1594-1597
Main Authors: Lokuta, M A, McDowell, M A, Paulnock, D M
Format: Article
Language:English
Subjects:
Animals
Bone Marrow Cells - metabolism
Cell Adhesion - immunology
Cell Differentiation - immunology
Cell Line
DNA-Binding Proteins - biosynthesis
DNA-Binding Proteins - genetics
DNA-Binding Proteins - isolation & purification
Immunoblotting
Isomerism
Macrophages - cytology
Macrophages - metabolism
Macrophages, Peritoneal - metabolism
Mice
Mice, Inbred BALB C
Milk Proteins
Molecular Weight
RNA, Messenger - biosynthesis
STAT5 Transcription Factor
Trans-Activators - biosynthesis
Trans-Activators - genetics
Trans-Activators - isolation & purification
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Summary:We are interested in understanding the molecular basis of macrophage (Mphi) differentiation and activation by cytokines. Recent reports have suggested that the transcription factor STAT5 may play a role in Mphi differentiation. In the experiments described here, we assessed the expression of STAT5-related molecules in three Mphi cell lines, RAW 264.7, WEHI-3, and WEHI-3D+, which represent different stages of Mphi maturation, and also in primary peritoneal and bone marrow Mphi from BALB/c mice. The studies revealed that the previously characterized STAT5a and STAT5b isoforms are detectable at both the mRNA and protein levels in these Mphi populations. Additional STAT5-related proteins were detected by immunoblot analysis and were preferentially expressed in both the immature WEHI-3 cell population and the adherent bone marrow population containing immature Mphi. These results identify new isoforms of STAT5 and demonstrate that distinct patterns of expression of STAT5-related proteins are observed in Mphi at different stages of maturation.
ISSN:0022-1767