β-Adrenergic agonist-induced nitrite production in isolated pig ciliary processes

We performed an experiment to assess whether in ciliary processes adrenergic-receptor agents can modulate the production of nitrite, an oxidized metabolite of nitric oxide (NO). The porcine ciliary processes and the iris were dissected and stored at -20 degrees C. Later, tissues were thawed, and nit...

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Bibliographic Details
Published in:Graefe's archive for clinical and experimental ophthalmology 1998-08, Vol.236 (8), p.613-616
Main Authors: LIU, R, FLAMMER, J, LÜSCHER, T. F, HAEFLIGER, I. O
Format: Article
Language:English
Subjects:
Adrenergic beta-Agonists - pharmacology
Adrenergic beta-Antagonists - pharmacology
Animals
Biological and medical sciences
Ciliary Body - drug effects
Ciliary Body - metabolism
Enzyme Inhibitors - pharmacology
Eye and associated structures. Visual pathways and centers. Vision
Fundamental and applied biological sciences. Psychology
NG-Nitroarginine Methyl Ester - pharmacology
Nitric Oxide - biosynthesis
Nitric Oxide Synthase - antagonists & inhibitors
Nitrites - metabolism
Norepinephrine - antagonists & inhibitors
Norepinephrine - pharmacology
Propranolol - pharmacology
Receptors, Adrenergic, beta - metabolism
Swine
Vertebrates: nervous system and sense organs
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Summary:We performed an experiment to assess whether in ciliary processes adrenergic-receptor agents can modulate the production of nitrite, an oxidized metabolite of nitric oxide (NO). The porcine ciliary processes and the iris were dissected and stored at -20 degrees C. Later, tissues were thawed, and nitrite measured by Griess reaction, before and 2 h after exposure to various drugs. In the ciliary processes, but not in the iris, the alpha-, beta-adrenergic receptor agonist, norepinephrine (0.01-10 microM), increased nitrite production (95 +/- 12%, P < 0.01). The norepinephrine-induced (0.1 microM) nitrite production was inhibited by the inhibitor of NO synthase, N omega-nitro-L-arginine methyl ester (0.5 mM; P < 0.05) and by the non-selective beta-adrenergic receptor antagonist, propranolol (10 microM; P < 0.01), but not by the non-selective alpha-adrenergic receptor antagonist, phentolamine (10 microM). In isolated porcine ciliary processes, beta-adrenergic receptor stimulation increases NO production.
ISSN:0721-832X
1435-702X
DOI:10.1007/s004170050130