Vasoactive intestinal peptide stimulates testosterone production by cultured fetal rat testicular cells

As a part of a series of studies on the regulation of testicular steroidogenesis in the fetal rat, we found that VIP stimulated fetal testicular cAMP production at a dose of 10 −9 mol/l, while a dose as low as 10 −12 mol/l stimulated testosterone production. RT-PCR analysis could not reveal either V...

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Bibliographic Details
Published in:Molecular and cellular endocrinology 1998-05, Vol.140 (1), p.175-178
Main Authors: El-Gehani, Faraj, Tena-Sempere, Manuel, Huhtaniemi, Ilpo
Format: Article
Language:English
Subjects:
Animals
Blotting, Southern
Brain - embryology
Brain - metabolism
Cells, Cultured
Chorionic Gonadotropin - pharmacology
Cyclic AMP - metabolism
Leydig cells
Leydig Cells - drug effects
Leydig Cells - metabolism
Male
Radioimmunoassay
Rats
Receptors, Vasoactive Intestinal Peptide - genetics
Receptors, Vasoactive Intestinal Peptide, Type II
Receptors, Vasoactive Intestinal Polypeptide, Type I
Reverse Transcriptase Polymerase Chain Reaction
Testis - embryology
Testis - metabolism
Testosterone
Testosterone - metabolism
Vasoactive intestinal peptide
Vasoactive Intestinal Peptide - blood
Vasoactive Intestinal Peptide - genetics
Vasoactive Intestinal Peptide - pharmacology
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Summary:As a part of a series of studies on the regulation of testicular steroidogenesis in the fetal rat, we found that VIP stimulated fetal testicular cAMP production at a dose of 10 −9 mol/l, while a dose as low as 10 −12 mol/l stimulated testosterone production. RT-PCR analysis could not reveal either VIP mRNA in fetal tissues or VIP1 receptor mRNA in the fetal or newborn testes, while VIP2 receptor mRNA was detected in fetal testes as early as E15.5. The testicular VIP content was unmeasurable by our radioimmunoassay method (
ISSN:0303-7207
1872-8057
DOI:10.1016/S0303-7207(98)00047-1