Nicotine stereoisomers and cotinine stimulate prostaglandin E2 but inhibit thromboxane B2 and leukotriene E4 synthesis in whole blood

The effects of (-)-nicotine (0.0005-500 microM), (+)-nicotine (0.0005-50 microM) and (-)-cotinine (0.0005-500 microM) on arachidonic acid metabolism were investigated in Ca2+ ionophore A23187 (calcimycin)-stimulated human whole blood in vitro. (-)-Nicotine and (-)-cotinine stimulated prostaglandin E...

Full description

Saved in:
Bibliographic Details
Published in:European journal of pharmacology 1998-07, Vol.353 (1), p.87-92
Main Authors: SAAREKS, V, MUCHA, I, SIEVI, E, VAPAATALO, H, RIUTTA, A
Format: Article
Language:English
Subjects:
Adult
Arachidonate 5-Lipoxygenase - blood
Arachidonic Acid - metabolism
Biological and medical sciences
Calcimycin - pharmacology
Cotinine - pharmacology
Dinoprostone - biosynthesis
Dinoprostone - blood
Humans
In Vitro Techniques
Ionophores - pharmacology
Leukotriene E4 - biosynthesis
Leukotriene E4 - blood
Medical sciences
Middle Aged
Nicotine - chemistry
Nicotine - pharmacology
Prostaglandin-Endoperoxide Synthases - blood
Stereoisomerism
Thromboxane B2 - biosynthesis
Thromboxane B2 - blood
Tobacco, tobacco smoking
Toxicology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The effects of (-)-nicotine (0.0005-500 microM), (+)-nicotine (0.0005-50 microM) and (-)-cotinine (0.0005-500 microM) on arachidonic acid metabolism were investigated in Ca2+ ionophore A23187 (calcimycin)-stimulated human whole blood in vitro. (-)-Nicotine and (-)-cotinine stimulated prostaglandin E2 but inhibited thromboxane B2 synthesis, as has been observed previously in A23187-stimulated polymorphonuclear leukocytes and platelet-rich plasma [Saareks, V., Riutta, A., Mucha, I., Alanko, J., Vapaatalo, H., 1993. Nicotine and cotinine modulate eicosanoid production in human leukocytes and platelet rich plasma. Eur. J. Pharmacol., 248, 345-349.]. (+)-Nicotine also stimulated prostaglandin E2 but inhibited thromboxane B2 synthesis. High concentrations of (-)-nicotine and (-)-cotinine and even nanomolar concentrations of (+)-nicotine inhibited leukotriene E4 synthesis. These results indicate that (-)-nicotine and (-)-cotinine stimulate cyclooxygenase but inhibit thromboxane synthase and 5-lipoxygenase in whole blood in vitro. (+)-Nicotine is capable of affecting in the same direction as well.
ISSN:0014-2999
1879-0712
DOI:10.1016/s0014-2999(98)00384-7