TNF-alpha released by comigrating monocytes promotes transendothelial migration of activated lymphocytes

We investigated mechanisms that increase motility and transendothelial trafficking of activated lymphocytes. Freshly isolated lymphocytes stimulated with immobilized anti-CD3 for 2 h migrate into polymerized collagen in 1.99+/-0.25-fold greater numbers and across confluent endothelial monolayers in...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of immunology (1950) 1998-09, Vol.161 (5), p.2481-2489
Main Authors: Green, D M, Trial, J, Birdsall, H H
Format: Article
Language:English
Subjects:
Antigens, CD - biosynthesis
CD3 Complex - immunology
Cell Adhesion - immunology
Cell Adhesion Molecules - biosynthesis
Cell Movement - drug effects
Cell Movement - immunology
Endothelium, Vascular - cytology
Endothelium, Vascular - immunology
Endothelium, Vascular - metabolism
Humans
Immune Sera - pharmacology
Interleukin-1 - biosynthesis
Interleukin-6 - biosynthesis
Lymphocyte Activation - immunology
Lymphocytes - immunology
Lymphocytes - physiology
Monocytes - metabolism
Monocytes - physiology
Receptors, Tumor Necrosis Factor - biosynthesis
Receptors, Tumor Necrosis Factor, Type I
Tumor Necrosis Factor-alpha - biosynthesis
Tumor Necrosis Factor-alpha - metabolism
Tumor Necrosis Factor-alpha - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We investigated mechanisms that increase motility and transendothelial trafficking of activated lymphocytes. Freshly isolated lymphocytes stimulated with immobilized anti-CD3 for 2 h migrate into polymerized collagen in 1.99+/-0.25-fold greater numbers and across confluent endothelial monolayers in 4.8+/-0.5-fold greater numbers compared with leukocytes incubated with non-specific IgG. Activated lymphocytes form clusters with monocytes, and their increased motility was dependent on the presence of comigrating monocytes. Five lines of evidence support the idea that monocytes modulate lymphocyte motility through the release of TNF-alpha: 1) flow-cytometric analyses, using highly specific and avid mAbs to probe permeabilized whole blood leukocytes, showed that >80% of circulating monocytes contain intracellular TNF-alpha, whereas
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.161.5.2481