Determination of right ventricular structure and function in normoxic and hypoxic mice : A transesophageal echocardiographic study

Noninvasive cardiac evaluation is of great importance in transgenic mice. Transthoracic echocardiography can visualize the left ventricle well but has not been as successful for the right ventricle (RV). We developed a method of transesophageal echocardiography (TEE) to evaluate murine RV size and f...

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Bibliographic Details
Published in:Circulation (New York, N.Y.) N.Y.), 1998-09, Vol.98 (10), p.1015-1021
Main Authors: SCHERRER-CROSBIE, M, STEUDEL, W, HUNZIKER, P. R, FOSTER, G. P, GARRIDO, L, LIEL-COHEN, N, ZAPOL, W. M, PICARD, M. H
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cardiac Output
Cardiovascular system
Echocardiography, Transesophageal - methods
Heart Ventricles - cytology
Heart Ventricles - pathology
Hemodynamics - physiology
Hypoxia - pathology
Hypoxia - physiopathology
Image Processing, Computer-Assisted
Investigative techniques, diagnostic techniques (general aspects)
Magnetic Resonance Imaging
Medical sciences
Mice
Myocardium - cytology
Myocardium - pathology
Reference Values
Reproducibility of Results
Stroke Volume
Ultrasonic investigative techniques
Ventricular Function, Right
Videotape Recording
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Summary:Noninvasive cardiac evaluation is of great importance in transgenic mice. Transthoracic echocardiography can visualize the left ventricle well but has not been as successful for the right ventricle (RV). We developed a method of transesophageal echocardiography (TEE) to evaluate murine RV size and function. Normoxic and chronically hypoxic mice (F(IO2)=0.11, 3 weeks) and agarose RV casts were scanned with a rotating 3.5F/30-MHz intravascular ultrasound probe. In vivo, the probe was inserted in the mouse esophagus and withdrawn to obtain contiguous horizontal planes at 1-mm intervals. In vitro, the probe was withdrawn along the left ventricular posterior wall of excised hearts. The borders of the RV were traced on each plane, allowing calculation of diastolic and systolic volumes, RV mass, RV ejection fraction, stroke volume, and cardiac output. RV wall thickness was measured. Echo volumes obtained in vitro were compared with cast volumes. Echo-derived cardiac output was compared with measurements of an ascending aortic Doppler flow probe. Echo-derived RV free wall mass was compared with true RV free wall weight. There was excellent agreement between cast and TEE volumes (y=0.82x+6.03, r=0.88, P
ISSN:0009-7322
1524-4539
DOI:10.1161/01.CIR.98.10.1015