Peptide Sequence of an Antibiotic Cecropin from the Vector Mosquito,Aedes albopictus

We have identified a 35-amino acid antibiotic cecropin secreted by an established mosquito cell line. C7-10 cells from the vector mosquito,Aedes albopictus,were incubated with heat-killedEscherichia coli,and materials secreted into the cell culture supernatant were recovered by acid precipitation. F...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1998-08, Vol.249 (2), p.410-415
Main Authors: Sun, Dongxu, Eccleston, Eric D., Fallon, Ann M.
Format: Article
Language:English
Subjects:
Aedes - metabolism
Aedes albopictus
Amino Acid Sequence
AMINO ACID SEQUENCES
Animals
Anti-Infective Agents - chemistry
Antimicrobial Cationic Peptides
cecropins
CELL CULTURE
Cell Line
CELL LINES
CHEMICAL COMPOSITION
Chromatography, High Pressure Liquid
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
Culicidae
CULTIVO DE CELULAS
CULTURE DE CELLULE
Diptera - chemistry
DISEASE VECTORS
Drosophila melanogaster
Drosophila melanogaster - chemistry
Escherichia coli
immunity factors
insect
Mass Spectrometry
MOLECULAR SEQUENCE DATA
mosquito
Peptide Fragments - chemistry
Peptides - chemistry
Sequence Homology
SWISSPROT/P81417
VECTEUR DE MALADIE
VECTORES
VECTORS
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Description
Summary:We have identified a 35-amino acid antibiotic cecropin secreted by an established mosquito cell line. C7-10 cells from the vector mosquito,Aedes albopictus,were incubated with heat-killedEscherichia coli,and materials secreted into the cell culture supernatant were recovered by acid precipitation. Following batch elution from Sep-Pak C18 cartridges and further purification by reverse phase high performance liquid chromatography (RP-HPLC) a predominant peak of antibacterial activity was characterized by mass spectrometry, amino acid composition analysis, and Edman degradation, yielding the sequence GGLKKLGKKLEGVGKRVFKASEKALPVAVGIKALG. Unlike other cecropins, the peptide was not amidated at the C-terminus.Aedes albopictusCecropin A (AalCecA) is the first cecropin to be described from a mosquito vector of human disease. Consistent with the classification of mosquitoes among the Dipteran suborder Nematocera, AalCecA shares only 36% amino acid identity with cecropins fromDrosophila melanogasterand other Cyclorrhaphid flies, whose mature cecropins share 80% to 100% amino acid identity.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1998.9150