Immunohistochemical Localization of Renin in Luteinizing Hormone-Producing Cells of Rat Pituitary

The location of renin (EC 3.4.99.19) in rat pituitary was determined by the peroxidase-antiperoxidase immunohistochemical technique. By using antisera prepared with purified rat renal renin, an immunoreactive substance was localized within ovoid cells scattered throughout the anterior pituitary. The...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1981-12, Vol.78 (12), p.7579-7583
Main Authors: Naruse, Kiyoko, Takii, Yukio, Inagami, Tadashi
Format: Article
Language:English
Subjects:
Adrenocorticotropic Hormone - metabolism
Animals
Anterior pituitary
Antibodies
Antigens
Antiserum
Cells
Cellular immunity
Growth Hormone - metabolism
Hormones
Immunoenzyme Techniques
Luteinization
Luteinizing Hormone - biosynthesis
Male
Pituitary Gland, Anterior - cytology
Pituitary Gland, Anterior - metabolism
Prolactin - metabolism
Rats
Rats, Inbred Strains
Renin - metabolism
Sheep
Thyrotropin - metabolism
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Summary:The location of renin (EC 3.4.99.19) in rat pituitary was determined by the peroxidase-antiperoxidase immunohistochemical technique. By using antisera prepared with purified rat renal renin, an immunoreactive substance was localized within ovoid cells scattered throughout the anterior pituitary. These cells were shown to be luteinizing hormone-producing cells by staining with anti-luteinizing hormone antisera in adjacent sections. By using the double staining method, the renin-containing cells were differentiated from cells containing corticotropin, thyrotropin, growth hormone (somatotropin), and prolactin (mammotropin). These results suggest a possible local role for renin in the anterior pituitary.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.78.12.7579