Detection of Enterocytozoon bieneusi in Two Human Immunodeficiency Virus—Negative Patients with Chronic Diarrhea by Polymerase Chain Reaction in Duodenal Biopsy Specimens and Review

Intestinal microsporidiosis has been associated traditionally with severely immunocompromised patients with AIDS. We describe two new cases of intestinal microsporidiosis due to Enterocytozoon bieneusi in human immunodeficiency virus–negative adults. Both patients presented with chronic nonbloody di...

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Bibliographic Details
Published in:Clinical infectious diseases 1998-08, Vol.27 (2), p.394-398
Main Authors: Gainzarain, Juan Carlos, Canut, Andrés, Lozano, Matías, Labora, Alicia, Carreras, Francisco, Fenoy, Soledad, Navajas, Raquel, Pieniazek, Norman J., Silva, Alexandre J. da, Aguila, Carmen del
Format: Article
Language:English
Subjects:
Adult
Aged
AIDS/HIV
Animals
Biological and medical sciences
Biopsies
Biopsy
Chronic Disease
Diarrhea
Diarrhea - parasitology
DNA, Protozoan - isolation & purification
Duodenum - parasitology
Duodenum - pathology
Enterocytozoon
Enterocytozoon bieneusi
Female
HIV Seronegativity
Human viral diseases
Humans
Infections
Infectious diseases
Male
Medical sciences
Microsporida - genetics
Microsporida - isolation & purification
Microsporidia
Microsporidiosis
Microsporidiosis - diagnosis
Parasites
Polymerase Chain Reaction
Specimens
Spores
Viral diseases
Viral diseases of the lymphoid tissue and the blood. Aids
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Description
Summary:Intestinal microsporidiosis has been associated traditionally with severely immunocompromised patients with AIDS. We describe two new cases of intestinal microsporidiosis due to Enterocytozoon bieneusi in human immunodeficiency virus–negative adults. Both patients presented with chronic nonbloody diarrhea, and one had intestinal lymphangiectasia as well. Intestinal microsporidiosis was diagnosed by evaluation of stool samples, and the specific species was determined by use of polymerase chain reaction (PCR) in duodenal biopsy specimens. To our knowledge, this is the first report of confirmation of E. bieneusi in the intestinal epithelium of HIV-negative individuals by use of PCR in duodenal biopsy specimens. Cases of intestinal microsporidiosis in HIV-negative individuals reported in the English-language literature are reviewed. These two new cases along with those described previously corroborate the need to evaluate for microsporidia in HIV-negative individuals with unexplained diarrhea.
ISSN:1058-4838
1537-6591
DOI:10.1086/514660