Uptake of poliovirus into the endosomal system of HeLa cells

To understand the topology and mechanism of poliovirus uncoating, the question of whether intact virions can be endocytosed by the host cell was studied by a combination of various techniques. In order to prevent alteration of the virus to subviral particles, Hela cells were infected at 26 °C. At th...

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Bibliographic Details
Published in:Archives of virology 1998-01, Vol.143 (7), p.1417-1424
Main Authors: Kronenberger, P, Schober, D, Prchla, E, Ofori-Anyinam, O, Vrijsen, R, Rombaut, B, Blaas, D, Fuchs, R, Boeyé, A
Format: Article
Language:English
Subjects:
Biological and medical sciences
electrophoresis
Endocytosis - physiology
endosomes
Endosomes - physiology
Endosomes - virology
Fundamental and applied biological sciences. Psychology
HeLa Cells
Horseradish Peroxidase
Humans
Membrane Proteins
Microbiology
plasma membrane
Poliovirus - pathogenicity
Poliovirus - physiology
Receptors, Virus - physiology
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
RNA
RNA, Viral - metabolism
Subcellular Fractions - virology
Temperature
virion
Virology
viruses
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Summary:To understand the topology and mechanism of poliovirus uncoating, the question of whether intact virions can be endocytosed by the host cell was studied by a combination of various techniques. In order to prevent alteration of the virus to subviral particles, Hela cells were infected at 26 °C. At this temperature the majority of cell-associated virions remained at the plasma membrane, whereas a smaller amount accumulated in vesicles having the same mobility (upon free-flow electrophoresis) and migration behaviour on Nycodenz density gradients as early and late endosomes. Co-localization of native poliovirions with endosomal markers was verified by peroxidase-induced diaminobenzidine density-shift of endosomal vesicles. Internalization of poliovirions into endosomes makes it likely, but does not prove that viral RNA can be released into the cytoplasm from the vesicular compartment.
ISSN:0304-8608
1432-8798
DOI:10.1007/s007050050385