An N-terminal region of LukF of staphylococcal leukocidin/gamma-hemolysin crucial for the biological activity of the toxin

The two staphylococcal bi-component toxins, leukocidin and gamma-hemolysin share LukF. This report identifies the pivotal amino acid residues in the N-terminal region of Lukf for the leukocytolytic and hemolytic activities in the presence of LukS and Hlg2, respectively, measuring the toxin activity...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1998-07, Vol.62 (7), p.1465-1467
Main Authors: Kaneko, J. (Tohoku Univ., Sendai (Japan). Faculty of Agriculture), Mascarenas, Ma.A.L, Huda, Md.N, Tomita, T, Kamio, Y
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacterial Proteins
Bacterial Toxins - chemistry
Bacterial Toxins - genetics
Bacteriology
Biological and medical sciences
Erythrocytes - metabolism
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Hemolysin Proteins - genetics
Humans
leukocidin
Leukocidins - chemistry
Leukocidins - genetics
LukF
Microbiology
Molecular Sequence Data
Mutation - genetics
N-terminal region
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
STAPHYLOCOCCUS AUREUS
Staphylococcus aureus - genetics
Staphylococcus aureus - metabolism
TOXINAS
TOXINE
TOXINS
γ-hemolysin
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Summary:The two staphylococcal bi-component toxins, leukocidin and gamma-hemolysin share LukF. This report identifies the pivotal amino acid residues in the N-terminal region of Lukf for the leukocytolytic and hemolytic activities in the presence of LukS and Hlg2, respectively, measuring the toxin activity of a series of Lukf mutants with truncated N-terminals. The data obtained showed that the Lukf mutant TF21, lacking 20 amino acid residues at the N-terminus of Lukf, failed to have any hemolytic activity and had less 10% leukocytolytic activity than that of the intact Lukf, while 16-residue truncations retained both toxin activities without loss. The Lukf mutants lacking 18- through 19-residue segments from the N-terminus showed low toxin activity on both target cells. All mutants having no toxin activity were also not capable of binding to the human erythrocytes. It can thus be concluded that the 3-residue segment, L18 Y19 K20 of Lukf is crucial for the biological activity of the toxin
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.62.1465