Osteogenic and osteoclastic cell interaction: development of a co-culture system

The processes involved in the regulation of bone cell metabolism are complex, including those implicated in bone cell coupling. This study was undertaken to develop a model that would permit real-time interaction between osteoclastic cells and osteoblasts in vitro. Osteogenic bone marrow stromal cel...

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Bibliographic Details
Published in:Cell and tissue research 1998-10, Vol.294 (1), p.99-108
Main Authors: Loomer, P M, Ellen, R P, Tenenbaum, H C
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - metabolism
Animals
Bone Resorption - metabolism
Cell Communication - physiology
Cellular Senescence
Chick Embryo
Chickens
Coculture Techniques
Collagen - metabolism
Culture Media, Conditioned
Diphosphonates - pharmacology
Microscopy, Electron, Scanning
Minerals - metabolism
Models, Biological
Osteoblasts - cytology
Osteoblasts - drug effects
Osteoblasts - metabolism
Osteoclasts - cytology
Osteoclasts - drug effects
Osteoclasts - metabolism
Osteogenesis - drug effects
Osteogenesis - physiology
Pamidronate
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Summary:The processes involved in the regulation of bone cell metabolism are complex, including those implicated in bone cell coupling. This study was undertaken to develop a model that would permit real-time interaction between osteoclastic cells and osteoblasts in vitro. Osteogenic bone marrow stromal cells were isolated from 18-day-old embryonic chickens, while osteoclastic cells were isolated from laying White Leghorn hens on calcium-deficient diets. Osteoclastic cells (5x10(5)) were seeded onto mineral thin films and suspended above osteogenic cells (1x10(4)) already plated on the bottoms of tissue culture plate wells. The data showed that after 4 days of incubation there was up to a fivefold (P
ISSN:0302-766X
1432-0878
DOI:10.1007/s004410051160