Ostrich microsatellite polymorphisms at the VIAS-OS4, VIAS-OS8, VIAS-OS14, VIAS-OS22, and VIAS-OS29 loci

Source/description: Five AC repeat microsatellites were isolated from an ostrich (Struthio camelus) genomic DNA library constructed in pUC18. Sau3A size selected fragments (between 220 and 460 bp) were screened with a (CA) sub(n).(GT) sub(n) probe and polymerase chain reaction (PCR) primers were des...

Full description

Saved in:
Bibliographic Details
Published in:Animal genetics 1998-08, Vol.29 (4), p.331-331
Main Authors: Ward, W K, McPartlan, H C, Matthews, M E, Robinson, N A
Format: Article
Language:English
Subjects:
Alleles
Animals
Birds - genetics
Chromosome Mapping - veterinary
Chromosomes - chemistry
Gene Frequency
Gene Library
Microsatellite Repeats - genetics
Molecular Sequence Data
Polymorphism, Genetic
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Source/description: Five AC repeat microsatellites were isolated from an ostrich (Struthio camelus) genomic DNA library constructed in pUC18. Sau3A size selected fragments (between 220 and 460 bp) were screened with a (CA) sub(n).(GT) sub(n) probe and polymerase chain reaction (PCR) primers were designed from the sequence of positive clones. These repeats were in the form of (GTGTAT) sub(2)(GT) sub(9)(GCGT) sub(4)(GT) sub(17) for VIAS-OS4 (EMBL AJ002877), (AC) sub(11) for VIAS-OS8 (GenBank U22053), (AC) sub(21) for VIAS-OS14 (GenBank U22435), (TG) sub(2)TA(TG) sub(7)TTTA(TG) sub(4)CA(TG) sub(4) for VIAS-OS22 (EMBL AJ002878), and (AC) sub(13)GG(AC) sub(6)GG(AC) sub(4) for VIAS-OS29 (EMBL AJ003011).
ISSN:0268-9146