Up-regulation of osteonectin/SPARC in age-related cataractous human lens epithelia

To characterize gene expression patterns between epithelia isolated from cataractous and normal human lenses. Reverse transcriptase differential display was used to identify differential expression between cataractous and normal epithelia. RT-PCR was used to compare pooled and individual RNA samples...

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Bibliographic Details
Published in:Molecular vision 1998-09, Vol.4, p.17-17
Main Authors: Kantorow, M, Horwitz, J, Carper, D
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Base Sequence
Cataract - metabolism
Female
Humans
Lens, Crystalline - metabolism
Male
Middle Aged
Molecular Sequence Data
Osteonectin - metabolism
Polymerase Chain Reaction
RNA - analysis
Sequence Homology, Nucleic Acid
Up-Regulation
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Summary:To characterize gene expression patterns between epithelia isolated from cataractous and normal human lenses. Reverse transcriptase differential display was used to identify differential expression between cataractous and normal epithelia. RT-PCR was used to compare pooled and individual RNA samples. One transcript, up-regulated in cataractous as compared to normal epithelia, was identified as osteonectin which is also known as SPARC (secreted acidic protein rich in cysteines). RT-PCR confirmed over-expression of this RNA. High levels of osteonectin mRNA were also detected in six individual epithelia dissected from cataractous lenses. The present study provides evidence for up-regulation of osteonectin in human age-related cataract and suggests that osteonectin, a protein involved in cell-cycle control, extracellular matrix and Ca++ binding, plays an important role in human lens homeostasis and may be involved in processes leading to lens opacity.
ISSN:1090-0535