Activated platelets induce monocyte chemotactic protein-1 secretion and surface expression of intercellular adhesion molecule-1 on endothelial cells

• Published in: Circulation (New York, N.Y.) N.Y.), 1998-09, Vol.98 (12), p.1164-1171
• Main Authors: GAWAZ, M, NEUMANN, F.-J, BRAND, K, DICKFELD, T, KOCH, W, LAUGWITZ, K.-L, ADELSBERGER, H, LANGENBRINK, K, PAGE, S, NEUMEIER, D, SCHÖMIG, A
• Format: Article
• Language: English
• Subjects: Arteriosclerosis - etiology; Arteriosclerosis - therapy; Atherosclerosis (general aspects, experimental research); Biological and medical sciences; Blood and lymphatic vessels; Blood Platelets - physiology; Cardiology. Vascular system; Cells, Cultured; Chemokine CCL2 - metabolism; Endothelium, Vascular - cytology; Endothelium, Vascular - metabolism; Humans; Intercellular Adhesion Molecule-1 - biosynthesis; Intercellular Adhesion Molecule-1 - genetics; Medical sciences; NF-kappa B - physiology; Platelet Activation; Promoter Regions, Genetic
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/01.cir.98.12.1164

Platelet/endothelium interaction plays an important role in the pathophysiology of inflammation and atherosclerosis. The role of platelets for monocyte chemotactic protein-1 (MCP-1) secretion and surface expression of intercellular adhesion molecule-1 (ICAM-1) on endothelial cells has been assessed. Monolayers of human umbilical vein endothelial cells were incubated with nonstimulated or ADP-activated platelets for 6 hours, and secretion of MCP-1 and surface expression of ICAM-1 were determined by ELISA and flow cytometry, respectively. In the presence of ADP-activated platelets, both MCP-1 secretion and ICAM-1 surface expression were significantly increased compared with nonstimulated platelets (P