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Electric field-induced changes in agonist-stimulated calcium fluxes of human HL-60 leukemia cells
The mechanism of biological effects of extremely‐low‐frequency electric and magnetic fields may involve induced changes of Ca2+ transport through plasma membrane ion channels. In this study we investigated the effects of externally applied, low‐intensity 60 Hz electric (E) fields (0.5 V/m, current d...
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Published in: | Bioelectromagnetics 1998, Vol.19 (6), p.366-376 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites |
Online Access: | Get full text |
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Summary: | The mechanism of biological effects of extremely‐low‐frequency electric and magnetic fields may involve induced changes of Ca2+ transport through plasma membrane ion channels. In this study we investigated the effects of externally applied, low‐intensity 60 Hz electric (E) fields (0.5 V/m, current density 0.8 A/m2+) on the agonist‐induced Ca2+ fluxes of HL‐60 leukemia cells. The suspensions of HL‐60 cells received E‐field or sham exposure for 60 min and were simultaneously stimulated either by 1 μM ATP or by 100 μM histamine or were not stimulated at all. After E‐field or sham exposure, the responses of the intracellular calcium levels of the cells to different concentrations of ATP (0.2–100 μM) were assessed. Compared with control cells, exposure of ATP‐activated cells to an E‐field resulted in a 20–30% decrease in the magnitude of [Ca2+]i elevation induced by a low concentration of ATP ( |
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ISSN: | 0197-8462 1521-186X |
DOI: | 10.1002/(SICI)1521-186X(1998)19:6<366::AID-BEM4>3.0.CO;2-# |