Measurement of bronchoconstriction using whole-body plethysmograph: comparison of freely moving versus restrained guinea pigs

• Published in: Journal of pharmacological and toxicological methods 1998-04, Vol.39 (3), p.163-168
• Main Authors: Chong, B.T.Y., Agrawal, D.K., Romero, F.A., Townley, R.G.
• Format: Article
• Language: English
• Subjects: Aerosols; Airways; Animals; Asthma; Bronchoconstriction; Bronchoconstriction - drug effects; Bronchoconstriction - physiology; Dose-Response Relationship, Drug; Guinea Pigs; Histamine - administration & dosage; Histamine - pharmacology; Histamine response; Male; Movement; Plethysmography, Whole Body - methods; Pulmonary Function; Restraint, Physical; Whole-body Plethysmograph
• ISSN: 1056-8719; 1873-488X
• DOI: 10.1016/S1056-8719(98)00021-5

We have previously measured pulmonary function in guinea pigs using a double-chambered plethysmograph, however, the question remains regarding the accuracy of the double-chamber to gauge the long-term pulmonary function of late asthmatic response. This may be affected by confounding factors, such as stress on the animal and differences in size of the collar around the neck. Therefore, in this study we compared histamine-induced bronchoconstriction in the same guinea pigs using a single- versus a double-chambered body box. In the double-chambered body box, the specific airway resistance is proportional to time delay between thoracic and nasal flows and measured in cmH 2O · s. Whereas, in the single-chambered body box, PenH units (Enhanced Pause) reflect “effort of breathing.” This is measured as the pause between inspiration and expiration. Doubling concentrations of histamine (12.5–200 μg/ml dissolved in normal saline) were administered by DeVilbiss nebulizer for 1 min, followed by 1 min suction of residual drug in the chamber, and then the airway resistance was recorded by the computer for the following 3 min. There was a 15-min wash-out period between two doses of histamine. There was no statistically significant difference (p > 0.05) in the PC 100 values for histamine between the two methods, however, it was much easier to work with the single-chambered body box in terms of handling the animal and eliminating the possible influence of collar placement on the bronchoconstriction. In conclusion, the data suggests histamine challenges produce equivalent PC 100 data in both the double-chambered plethysmograph with sRAW units and single-chambered plethysmograph using the PenH units.