Influence of Verapamil and Digoxin on the in Vitro Binding of Doxorubicin to the Rat Heart

A study has been carried out to characterize the binding of doxorubicin to hear homogenates and subcellular fractions. The technique chosen to perform the study was equilibrium dilalysis and the levels of anthracycline in the samples obtained from the dialysis were assessed using HPLC. Doxorubicin h...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 1998/08/15, Vol.21(8), pp.839-843
Main Authors: ALVAREZ-CEDRON, Loreto, LOPEZ, Francisco G., LANAO, Jose M.
Format: Article
Language:English
Subjects:
Animals
Antibiotics, Antineoplastic - metabolism
Antineoplastic agents
binding
Biological and medical sciences
digoxin
Digoxin - pharmacology
doxorubicin
Doxorubicin - metabolism
General aspects
heart
Medical sciences
Myocardium - metabolism
Pharmacology. Drug treatments
Rats
Rats, Wistar
subcellular fraction
verapamil
Verapamil - pharmacology
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Summary:A study has been carried out to characterize the binding of doxorubicin to hear homogenates and subcellular fractions. The technique chosen to perform the study was equilibrium dilalysis and the levels of anthracycline in the samples obtained from the dialysis were assessed using HPLC. Doxorubicin has high affinity to heart homogenates and subcellular fractions such as nuclear, mitochondrial and microsomal. The binding was saturable and dose-dependent. Doxorubicin binding is decreased in the presence of digoxin and especially verapamil.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.21.839