New reference allelic ladders to improve allelic designation in a multiplex STR system

This paper reports the composition of a new reference allelic ladder mixture for use with a multiplex DNA profiling system consisting of six short tandem repeat loci. The loci included in this mixture are HUMTH01, D21S11, D18S51, D8S1179, HUMVWAF31/A, HUMFIBRA/FGA and an amelogenin sex test. Sequenc...

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Bibliographic Details
Published in:International journal of legal medicine 1998-08, Vol.111 (5), p.267-272
Main Authors: Griffiths, R A, Barber, M D, Johnson, P E, Gillbard, S M, Haywood, M D, Smith, C D, Arnold, J, Burke, T, Urquhart, A J, Gill, P
Format: Article
Language:English
Subjects:
Alleles
Fish
Forensic Medicine
Humans
Tandem Repeat Sequences - genetics
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Summary:This paper reports the composition of a new reference allelic ladder mixture for use with a multiplex DNA profiling system consisting of six short tandem repeat loci. The loci included in this mixture are HUMTH01, D21S11, D18S51, D8S1179, HUMVWAF31/A, HUMFIBRA/FGA and an amelogenin sex test. Sequence analysis of individual ladder alleles was carried out and allelic designations made in accordance with the recommendations of the International Society of Forensic Haemogenetics (1992; 1994). A series of rare alleles which increase the range of alleles previously reported were identified. By including some of the rare alleles into the ladder marker system, we have significantly improved the ability to identify new alleles in unknown samples.
ISSN:0937-9827
1437-1596
DOI:10.1007/s004140050167