In situ measurements of creatine kinase flux by NMR. The lessons from bioengineered mice

P-31 nuclear magnetic resonance (NMR) is uniquely suited to measure the kinetics of the phosphoryl-exchange reaction catalyzed by creatine kinase in intact mammalian tissue, especially striated muscle. Recently developed transgenic mouse models of the creatine kinase iso-enzyme system open novel opp...

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Bibliographic Details
Published in:Molecular and cellular biochemistry 1998-07, Vol.184 (1-2), p.195-208
Main Authors: Nicolay, K, van Dorsten, F A, Reese, T, Kruiskamp, M J, Gellerich, J F, van Echteld, C J
Format: Article
Language:English
Subjects:
Animals
Creatine Kinase - metabolism
Energy Metabolism - physiology
Enzymes
Fluctuations
In situ measurement
Isoenzymes
Kinases
Kinetics
Magnetic Resonance Spectroscopy - methods
Mice
Mice, Transgenic
Models, Genetic
Muscle, Skeletal - physiology
Myocardium - metabolism
NMR
Nuclear magnetic resonance
Quantitative research
Rodents
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Description
Summary:P-31 nuclear magnetic resonance (NMR) is uniquely suited to measure the kinetics of the phosphoryl-exchange reaction catalyzed by creatine kinase in intact mammalian tissue, especially striated muscle. Recently developed transgenic mouse models of the creatine kinase iso-enzyme system open novel opportunities to assess the functional importance of the individual iso-enzymes and their relative contribution to the total in situ flux through the CK reaction. This chapter reviews the most recent findings from NMR flux measurements on such genetic models of CK function. Findings in intact mouse skeletal and cardiac muscle in vivo are compared to data from purified mitochondrial and cytosolic creatine kinase in vitro. The relevance of findings in transgenic animals for the function of CK in wild-type tissue is described and the perspectives of transgenic techniques in future quantitative studies on the creatine kinase iso-enzyme system are indicated.
ISSN:0300-8177
1573-4919
DOI:10.1023/A:1006867818617