A physical map of Arabidopsis thaliana chromosome 3 represented by two contigs of CIC YAC, P1, TAC and BAC clones

We have constructed a physical map of Arabidopsis thaliana chromosome 3 by ordering the clones from CIC YAC, P1, TAC and BAC libraries using the sequences of a variety of genetic and EST markers and terminal sequences of clones. The markers used were 112 DNA markers, 145 YAC end sequences, and 156 e...

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Bibliographic Details
Published in:DNA research 1998, Vol.5 (3), p.163-168
Main Authors: Sato, S, Kotani, H, Hayashi, R, Liu, Y G, Shibata, D, Tabata, S
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Cloning, Molecular
Contig Mapping
DNA, Plant - genetics
Genetic Markers
Genomic Library
Physical Chromosome Mapping
Restriction Mapping
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Summary:We have constructed a physical map of Arabidopsis thaliana chromosome 3 by ordering the clones from CIC YAC, P1, TAC and BAC libraries using the sequences of a variety of genetic and EST markers and terminal sequences of clones. The markers used were 112 DNA markers, 145 YAC end sequences, and 156 end sequences of P1, TAC and BAC clones. The entire genome of chromosome 3, except for the centromeric and telomeric regions, was covered by two large contigs, 13.6 Mb and 9.2 Mb long. This physical map will facilitate map-based cloning experiments as well as genome sequencing of chromosome 3. The map and end sequence information are available on the KAOS (Kazusa Arabidopsis data Opening Site) web site at http://www.kazusa.or.jp/arabi/.
ISSN:1340-2838
DOI:10.1093/dnares/5.3.163