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Pituitary-dependent expression of the testicular angiotensin II receptor and its subtypes in rats
Angiotensin II (AT2) has been implicated in the growth and/or differentiation of its target tissues. In the present study, testicular AT2 receptor and its subtypes in hypophysectomized rats were examined using quantitative in vitro autoradiography and Northern blot analysis in an attempt to determin...
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| Published in: | International journal of andrology 1998-08, Vol.21 (4), p.177-185 |
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| Main Authors: | , , , , , , , , |
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| cited_by | cdi_FETCH-LOGICAL-c4812-33031d0f083ae4606b28c7b57999a7b81c28826f94b319436f503ff09feb34103 |
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| cites | cdi_FETCH-LOGICAL-c4812-33031d0f083ae4606b28c7b57999a7b81c28826f94b319436f503ff09feb34103 |
| container_end_page | 185 |
| container_issue | 4 |
| container_start_page | 177 |
| container_title | International journal of andrology |
| container_volume | 21 |
| creator | HIRAI, K SONG, K KANEHARA, H SHIOTA, N UEDA, H KIM, S MIYAZAKI, H KATSUOKA, Y MIYAZAKI, M |
| description | Angiotensin II (AT2) has been implicated in the growth and/or differentiation of its target tissues. In the present study, testicular AT2 receptor and its subtypes in hypophysectomized rats were examined using quantitative in vitro autoradiography and Northern blot analysis in an attempt to determine possible involvement of pituitary hormones in their expression. Prepubescent (3 weeks of age) male Sprague–Dawley rats underwent hypophysectomy or sham operation. From 10 days thereafter, they were treated with vehicle, growth hormone, human chorionic gonadotrophin or human menopausal gonadotrophin for 10 days. Testicular AT2 receptors were labelled with 125I‐[Sar1,Ile8] AT2 and differentiated into its subtypes (AT1 and FAT2) according to their susceptibility to AT1 (losartan, 5 μM) and AT2 (CGP42112B, 1 μM) antagonists. Hypophysectomy led to a marked increase in AT2 receptor concentration (sham‐operated rats: 0.7 ± 0.2 fmol/mg protein, hypophysectomized rats: 2.5 ± 0.6 fmol/mg protein, mean ± SEM, n = 11–12, p |
| doi_str_mv | 10.1046/j.1365-2605.1998.00102.x |
| format | article |
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In the present study, testicular AT2 receptor and its subtypes in hypophysectomized rats were examined using quantitative in vitro autoradiography and Northern blot analysis in an attempt to determine possible involvement of pituitary hormones in their expression. Prepubescent (3 weeks of age) male Sprague–Dawley rats underwent hypophysectomy or sham operation. From 10 days thereafter, they were treated with vehicle, growth hormone, human chorionic gonadotrophin or human menopausal gonadotrophin for 10 days. Testicular AT2 receptors were labelled with 125I‐[Sar1,Ile8] AT2 and differentiated into its subtypes (AT1 and FAT2) according to their susceptibility to AT1 (losartan, 5 μM) and AT2 (CGP42112B, 1 μM) antagonists. Hypophysectomy led to a marked increase in AT2 receptor concentration (sham‐operated rats: 0.7 ± 0.2 fmol/mg protein, hypophysectomized rats: 2.5 ± 0.6 fmol/mg protein, mean ± SEM, n = 11–12, p < 0.01) with predominant occurrence of AT1 receptors. Both human chorionic gonadotrophin and human menopausal gonadotrophin decreased testicular AT2 receptor concentration, whereas growth hormone did not affect AT2 receptor expression. Northern blot analysis revealed both testicular AT1 and AT2 receptor mRNA expression to be significantly increased after hypophysectomy and reduced by gonadotrophin treatment. These results suggest that the expression of testicular AT2 receptors is regulated by pituitary gonadotrophins and that AT2 may play a role in testicular growth and/or differentiation.</description><identifier>ISSN: 0105-6263</identifier><identifier>EISSN: 1365-2605</identifier><identifier>DOI: 10.1046/j.1365-2605.1998.00102.x</identifier><identifier>PMID: 9749347</identifier><identifier>CODEN: IJANDP</identifier><language>eng</language><publisher>Oxford UK: Blackwell Science Ltd</publisher><subject>Angiotensin II - metabolism ; angiotensin II receptor ; Animals ; Biological and medical sciences ; Body Weight ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Humans ; Hypophysectomy ; in vitro autoradiography ; Male ; Mammalian male genital system ; Morphology. Physiology ; Organ Size ; Pituitary Gland - physiology ; Rats ; Rats, Sprague-Dawley ; receptor subtypes ; Receptor, Angiotensin, Type 1 ; Receptor, Angiotensin, Type 2 ; Receptors, Angiotensin - genetics ; Receptors, Angiotensin - metabolism ; RNA, Messenger ; testis ; Testis - metabolism ; Vertebrates: reproduction</subject><ispartof>International journal of andrology, 1998-08, Vol.21 (4), p.177-185</ispartof><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4812-33031d0f083ae4606b28c7b57999a7b81c28826f94b319436f503ff09feb34103</citedby><cites>FETCH-LOGICAL-c4812-33031d0f083ae4606b28c7b57999a7b81c28826f94b319436f503ff09feb34103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2360528$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9749347$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HIRAI, K</creatorcontrib><creatorcontrib>SONG, K</creatorcontrib><creatorcontrib>KANEHARA, H</creatorcontrib><creatorcontrib>SHIOTA, N</creatorcontrib><creatorcontrib>UEDA, H</creatorcontrib><creatorcontrib>KIM, S</creatorcontrib><creatorcontrib>MIYAZAKI, H</creatorcontrib><creatorcontrib>KATSUOKA, Y</creatorcontrib><creatorcontrib>MIYAZAKI, M</creatorcontrib><title>Pituitary-dependent expression of the testicular angiotensin II receptor and its subtypes in rats</title><title>International journal of andrology</title><addtitle>Int J Androl</addtitle><description>Angiotensin II (AT2) has been implicated in the growth and/or differentiation of its target tissues. In the present study, testicular AT2 receptor and its subtypes in hypophysectomized rats were examined using quantitative in vitro autoradiography and Northern blot analysis in an attempt to determine possible involvement of pituitary hormones in their expression. Prepubescent (3 weeks of age) male Sprague–Dawley rats underwent hypophysectomy or sham operation. From 10 days thereafter, they were treated with vehicle, growth hormone, human chorionic gonadotrophin or human menopausal gonadotrophin for 10 days. Testicular AT2 receptors were labelled with 125I‐[Sar1,Ile8] AT2 and differentiated into its subtypes (AT1 and FAT2) according to their susceptibility to AT1 (losartan, 5 μM) and AT2 (CGP42112B, 1 μM) antagonists. Hypophysectomy led to a marked increase in AT2 receptor concentration (sham‐operated rats: 0.7 ± 0.2 fmol/mg protein, hypophysectomized rats: 2.5 ± 0.6 fmol/mg protein, mean ± SEM, n = 11–12, p < 0.01) with predominant occurrence of AT1 receptors. Both human chorionic gonadotrophin and human menopausal gonadotrophin decreased testicular AT2 receptor concentration, whereas growth hormone did not affect AT2 receptor expression. Northern blot analysis revealed both testicular AT1 and AT2 receptor mRNA expression to be significantly increased after hypophysectomy and reduced by gonadotrophin treatment. These results suggest that the expression of testicular AT2 receptors is regulated by pituitary gonadotrophins and that AT2 may play a role in testicular growth and/or differentiation.</description><subject>Angiotensin II - metabolism</subject><subject>angiotensin II receptor</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Body Weight</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Hypophysectomy</subject><subject>in vitro autoradiography</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Morphology. Physiology</subject><subject>Organ Size</subject><subject>Pituitary Gland - physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>receptor subtypes</subject><subject>Receptor, Angiotensin, Type 1</subject><subject>Receptor, Angiotensin, Type 2</subject><subject>Receptors, Angiotensin - genetics</subject><subject>Receptors, Angiotensin - metabolism</subject><subject>RNA, Messenger</subject><subject>testis</subject><subject>Testis - metabolism</subject><subject>Vertebrates: reproduction</subject><issn>0105-6263</issn><issn>1365-2605</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqNkE2P0zAQhi0EWkrhJyD5gLgl-CNx7AOH1Qp2u6yWD4HYm-U4Y3BJk2A7ov33OLTqmdOM5n3fGftBCFNSUlKJN9uSclEXTJC6pErJkhBKWLl_hFZn4TFa5WFdCCb4U_Qsxi0hhEtOL9CFairFq2aFzCefZp9MOBQdTDB0MCQM-ylAjH4c8Ohw-gk4QUzezr0J2Aw__JhgiH7Amw0OYGFK4zLvsE8Rx7lNhwkiznowKT5HT5zpI7w41TX69v7d16ub4u7j9ebq8q6wlaSs4Jxw2hFHJDdQCSJaJm3T1o1SyjStpJZJyYRTVcupqrhwNeHOEeWg5RUlfI1eH_dOYfw95_fqnY8W-t4MMM5RN1xlUvnbaySPRhvGGAM4PQW_ywQ0JXqhq7d6gagXiHqhq__R1fscfXm6Mbc76M7BE86svzrpJlrTu2AG6-PZxnjeyGS2vT3a_vgeDv99Xm9uL-9zl_PFMe9jgv05b8IvLRre1Pr7_bVmXz483D58lvqG_wURZ6S7</recordid><startdate>19980801</startdate><enddate>19980801</enddate><creator>HIRAI, K</creator><creator>SONG, K</creator><creator>KANEHARA, H</creator><creator>SHIOTA, N</creator><creator>UEDA, H</creator><creator>KIM, S</creator><creator>MIYAZAKI, H</creator><creator>KATSUOKA, Y</creator><creator>MIYAZAKI, M</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980801</creationdate><title>Pituitary-dependent expression of the testicular angiotensin II receptor and its subtypes in rats</title><author>HIRAI, K ; SONG, K ; KANEHARA, H ; SHIOTA, N ; UEDA, H ; KIM, S ; MIYAZAKI, H ; KATSUOKA, Y ; MIYAZAKI, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4812-33031d0f083ae4606b28c7b57999a7b81c28826f94b319436f503ff09feb34103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Angiotensin II - metabolism</topic><topic>angiotensin II receptor</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Body Weight</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Hypophysectomy</topic><topic>in vitro autoradiography</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Morphology. Physiology</topic><topic>Organ Size</topic><topic>Pituitary Gland - physiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>receptor subtypes</topic><topic>Receptor, Angiotensin, Type 1</topic><topic>Receptor, Angiotensin, Type 2</topic><topic>Receptors, Angiotensin - genetics</topic><topic>Receptors, Angiotensin - metabolism</topic><topic>RNA, Messenger</topic><topic>testis</topic><topic>Testis - metabolism</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HIRAI, K</creatorcontrib><creatorcontrib>SONG, K</creatorcontrib><creatorcontrib>KANEHARA, H</creatorcontrib><creatorcontrib>SHIOTA, N</creatorcontrib><creatorcontrib>UEDA, H</creatorcontrib><creatorcontrib>KIM, S</creatorcontrib><creatorcontrib>MIYAZAKI, H</creatorcontrib><creatorcontrib>KATSUOKA, Y</creatorcontrib><creatorcontrib>MIYAZAKI, M</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of andrology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HIRAI, K</au><au>SONG, K</au><au>KANEHARA, H</au><au>SHIOTA, N</au><au>UEDA, H</au><au>KIM, S</au><au>MIYAZAKI, H</au><au>KATSUOKA, Y</au><au>MIYAZAKI, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pituitary-dependent expression of the testicular angiotensin II receptor and its subtypes in rats</atitle><jtitle>International journal of andrology</jtitle><addtitle>Int J Androl</addtitle><date>1998-08-01</date><risdate>1998</risdate><volume>21</volume><issue>4</issue><spage>177</spage><epage>185</epage><pages>177-185</pages><issn>0105-6263</issn><eissn>1365-2605</eissn><coden>IJANDP</coden><abstract>Angiotensin II (AT2) has been implicated in the growth and/or differentiation of its target tissues. In the present study, testicular AT2 receptor and its subtypes in hypophysectomized rats were examined using quantitative in vitro autoradiography and Northern blot analysis in an attempt to determine possible involvement of pituitary hormones in their expression. Prepubescent (3 weeks of age) male Sprague–Dawley rats underwent hypophysectomy or sham operation. From 10 days thereafter, they were treated with vehicle, growth hormone, human chorionic gonadotrophin or human menopausal gonadotrophin for 10 days. Testicular AT2 receptors were labelled with 125I‐[Sar1,Ile8] AT2 and differentiated into its subtypes (AT1 and FAT2) according to their susceptibility to AT1 (losartan, 5 μM) and AT2 (CGP42112B, 1 μM) antagonists. Hypophysectomy led to a marked increase in AT2 receptor concentration (sham‐operated rats: 0.7 ± 0.2 fmol/mg protein, hypophysectomized rats: 2.5 ± 0.6 fmol/mg protein, mean ± SEM, n = 11–12, p < 0.01) with predominant occurrence of AT1 receptors. Both human chorionic gonadotrophin and human menopausal gonadotrophin decreased testicular AT2 receptor concentration, whereas growth hormone did not affect AT2 receptor expression. Northern blot analysis revealed both testicular AT1 and AT2 receptor mRNA expression to be significantly increased after hypophysectomy and reduced by gonadotrophin treatment. These results suggest that the expression of testicular AT2 receptors is regulated by pituitary gonadotrophins and that AT2 may play a role in testicular growth and/or differentiation.</abstract><cop>Oxford UK</cop><pub>Blackwell Science Ltd</pub><pmid>9749347</pmid><doi>10.1046/j.1365-2605.1998.00102.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | International journal of andrology, 1998-08, Vol.21 (4), p.177-185 |
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| language | eng |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Angiotensin II - metabolism angiotensin II receptor Animals Biological and medical sciences Body Weight Fundamental and applied biological sciences. Psychology Gene Expression Humans Hypophysectomy in vitro autoradiography Male Mammalian male genital system Morphology. Physiology Organ Size Pituitary Gland - physiology Rats Rats, Sprague-Dawley receptor subtypes Receptor, Angiotensin, Type 1 Receptor, Angiotensin, Type 2 Receptors, Angiotensin - genetics Receptors, Angiotensin - metabolism RNA, Messenger testis Testis - metabolism Vertebrates: reproduction |
| title | Pituitary-dependent expression of the testicular angiotensin II receptor and its subtypes in rats |
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