In Vitro Transfer of Reactivity to Trypanosoma cruzi Antigens from Rat Cells to Human Cells with Immune RNA

The immunologic activity of polysomal RNA isolated from the spleens of rats infected with Trypanosoma cruzi was assessed by the leukocyte migration-inhibition assay as an in vitro correlate of delayed hypersensitivity. This RNA preparation transferred reactivity to T. cruzi antigens to nonsensitized...

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Bibliographic Details
Published in:The Journal of infectious diseases 1982-02, Vol.145 (2), p.148-151
Main Authors: De Lucca, Fernando L., Bertolini, Maria C., Zini, Marcelo N.
Format: Article
Language:English
Subjects:
Animals
Antigens
Antigens - immunology
Blood
Cell Biology
Chagas Disease - immunology
Delayed hypersensitivity
Humans
Hypersensitivity, Delayed - immunology
Immunity, Maternally-Acquired - drug effects
Immunologic Techniques
In Vitro Techniques
Infections
Leukocytes
Lymphocytes
Lymphocytes - immunology
Mice
Rats
Reactivity
Ribonucleases - pharmacology
RNA
RNA - immunology
Spleen
Trypanosoma cruzi
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Summary:The immunologic activity of polysomal RNA isolated from the spleens of rats infected with Trypanosoma cruzi was assessed by the leukocyte migration-inhibition assay as an in vitro correlate of delayed hypersensitivity. This RNA preparation transferred reactivity to T. cruzi antigens to nonsensitized human lymphocytes from peripheral blood. This transfer of reactivity was abolished by treatment of the RNA preparation with ribonuclease. The transfer of delayed hypersensitivity in vitro from rat cells to human cells was antigen-specific. Polysomal RNA also conferred significant protection against infection with T. cruzi in mice, as evaluated by the level of parasitemia and the survival rate of mice challenged with virulent strain Y of T. cruzi.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/145.2.148