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Influence of calf serum on glucocorticoid-responses of certain progesterone derivatives
The following in vitro glucocorticoid (GC) parameters of progesterone (P), 1-ene progesterone (ΔP), 11 β-hydroxyprogesterone (HOP), 11 β-1-ene progesterone (ΔHOP) and dexamethasone (Dexa) were assayed in the presence or absence of bovine calf serum (BCS): binding to thymus cytosol, dissociation of t...
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| Published in: | The Journal of steroid biochemistry and molecular biology 1998-08, Vol.66 (4), p.211-216 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c389t-ed65408f6f734217e8ec904efba6a7bade29dfff76abe97e1740efcabc4de77f3 |
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| cites | cdi_FETCH-LOGICAL-c389t-ed65408f6f734217e8ec904efba6a7bade29dfff76abe97e1740efcabc4de77f3 |
| container_end_page | 216 |
| container_issue | 4 |
| container_start_page | 211 |
| container_title | The Journal of steroid biochemistry and molecular biology |
| container_volume | 66 |
| creator | Vicent, G.P. Burton, G. Ghini, A. Lantos, C.P. Galigniana, M.D. |
| description | The following
in vitro glucocorticoid (GC) parameters of progesterone (P), 1-ene progesterone (ΔP), 11
β-hydroxyprogesterone (HOP), 11
β-1-ene progesterone (ΔHOP) and dexamethasone (Dexa) were assayed in the presence or absence of bovine calf serum (BCS): binding to thymus cytosol, dissociation of the glucocorticoid receptor (GR)–heat shock protein 9O (hsp90) complex (diss.), tyrosine aminotransferase (TAT) induction in hepatocytes and the inhibition of
3H-uridine and
35S-methionine uptake by thymocytes. Without BCS, steroids were in most cases active in this general order: Dex>ΔHOP>HOP>ΔP>P. BCS abolished all activities in P and ΔP, but left them unaltered in all other steroids, except diss. in HOP, which diminished intermediately. Binding of P, ΔP, HOP and ΔHOP to GR and CBG paralleled their
in vivo activating effects on glycogen deposition. Conclusions: in this steroid series, BCS, but not CBG, inhibits GC responses of P and ΔP. 11-
β hydroxylation frees those molecules from the inhibitory effects of BCS. |
| doi_str_mv | 10.1016/S0960-0760(98)00040-5 |
| format | article |
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in vitro glucocorticoid (GC) parameters of progesterone (P), 1-ene progesterone (ΔP), 11
β-hydroxyprogesterone (HOP), 11
β-1-ene progesterone (ΔHOP) and dexamethasone (Dexa) were assayed in the presence or absence of bovine calf serum (BCS): binding to thymus cytosol, dissociation of the glucocorticoid receptor (GR)–heat shock protein 9O (hsp90) complex (diss.), tyrosine aminotransferase (TAT) induction in hepatocytes and the inhibition of
3H-uridine and
35S-methionine uptake by thymocytes. Without BCS, steroids were in most cases active in this general order: Dex>ΔHOP>HOP>ΔP>P. BCS abolished all activities in P and ΔP, but left them unaltered in all other steroids, except diss. in HOP, which diminished intermediately. Binding of P, ΔP, HOP and ΔHOP to GR and CBG paralleled their
in vivo activating effects on glycogen deposition. Conclusions: in this steroid series, BCS, but not CBG, inhibits GC responses of P and ΔP. 11-
β hydroxylation frees those molecules from the inhibitory effects of BCS.</description><identifier>ISSN: 0960-0760</identifier><identifier>EISSN: 1879-1220</identifier><identifier>DOI: 10.1016/S0960-0760(98)00040-5</identifier><identifier>PMID: 9744518</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adrenalectomy ; Animals ; Biological and medical sciences ; Blood ; Cattle ; Cell physiology ; Cells, Cultured ; Corticosterone - metabolism ; Cytosol - metabolism ; Dexamethasone - pharmacology ; Enzyme Induction ; Fundamental and applied biological sciences. Psychology ; Hormonal regulation ; HSP90 Heat-Shock Proteins - metabolism ; Liver - drug effects ; Liver - enzymology ; Liver - metabolism ; Liver Glycogen - biosynthesis ; Male ; Methionine - metabolism ; Mice ; Mice, Inbred BALB C ; Molecular and cellular biology ; Progesterone - analogs & derivatives ; Progesterone - pharmacology ; Rats ; Rats, Sprague-Dawley ; Receptors, Glucocorticoid - metabolism ; Structure-Activity Relationship ; Thymus Gland - drug effects ; Thymus Gland - metabolism ; Transcortin - metabolism ; Tyrosine Transaminase - biosynthesis ; Uridine - metabolism</subject><ispartof>The Journal of steroid biochemistry and molecular biology, 1998-08, Vol.66 (4), p.211-216</ispartof><rights>1998 Elsevier Science Ltd</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-ed65408f6f734217e8ec904efba6a7bade29dfff76abe97e1740efcabc4de77f3</citedby><cites>FETCH-LOGICAL-c389t-ed65408f6f734217e8ec904efba6a7bade29dfff76abe97e1740efcabc4de77f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2375379$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9744518$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vicent, G.P.</creatorcontrib><creatorcontrib>Burton, G.</creatorcontrib><creatorcontrib>Ghini, A.</creatorcontrib><creatorcontrib>Lantos, C.P.</creatorcontrib><creatorcontrib>Galigniana, M.D.</creatorcontrib><title>Influence of calf serum on glucocorticoid-responses of certain progesterone derivatives</title><title>The Journal of steroid biochemistry and molecular biology</title><addtitle>J Steroid Biochem Mol Biol</addtitle><description>The following
in vitro glucocorticoid (GC) parameters of progesterone (P), 1-ene progesterone (ΔP), 11
β-hydroxyprogesterone (HOP), 11
β-1-ene progesterone (ΔHOP) and dexamethasone (Dexa) were assayed in the presence or absence of bovine calf serum (BCS): binding to thymus cytosol, dissociation of the glucocorticoid receptor (GR)–heat shock protein 9O (hsp90) complex (diss.), tyrosine aminotransferase (TAT) induction in hepatocytes and the inhibition of
3H-uridine and
35S-methionine uptake by thymocytes. Without BCS, steroids were in most cases active in this general order: Dex>ΔHOP>HOP>ΔP>P. BCS abolished all activities in P and ΔP, but left them unaltered in all other steroids, except diss. in HOP, which diminished intermediately. Binding of P, ΔP, HOP and ΔHOP to GR and CBG paralleled their
in vivo activating effects on glycogen deposition. Conclusions: in this steroid series, BCS, but not CBG, inhibits GC responses of P and ΔP. 11-
β hydroxylation frees those molecules from the inhibitory effects of BCS.</description><subject>Adrenalectomy</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Cattle</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>Corticosterone - metabolism</subject><subject>Cytosol - metabolism</subject><subject>Dexamethasone - pharmacology</subject><subject>Enzyme Induction</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormonal regulation</subject><subject>HSP90 Heat-Shock Proteins - metabolism</subject><subject>Liver - drug effects</subject><subject>Liver - enzymology</subject><subject>Liver - metabolism</subject><subject>Liver Glycogen - biosynthesis</subject><subject>Male</subject><subject>Methionine - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular and cellular biology</subject><subject>Progesterone - analogs & derivatives</subject><subject>Progesterone - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, Glucocorticoid - metabolism</subject><subject>Structure-Activity Relationship</subject><subject>Thymus Gland - drug effects</subject><subject>Thymus Gland - metabolism</subject><subject>Transcortin - metabolism</subject><subject>Tyrosine Transaminase - biosynthesis</subject><subject>Uridine - metabolism</subject><issn>0960-0760</issn><issn>1879-1220</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqFkE1LAzEQhoMotVZ_QmEPInpYTfYrm5NI8aNQ8KDiMWSTiUS2SU12C_57s-3Sq6c5zPPOvDwIzQm-JZhUd2-YVTjFtMLXrL7BGBc4LY_QlNSUpSTL8DGaHpBTdBbCd4TynNAJmjBaFCWpp-hzaXXbg5WQOJ1I0eokgO_XibPJV9tLJ53vjHRGpR7CxtkAYUeC74Sxyca7LwgdeGchUeDNVnRmC-EcnWjRBrgY5wx9PD2-L17S1evzcvGwSmVesy4FVZUFrnWlaV5khEINkuECdCMqQRuhIGNKa00r0QCjQGiBQUvRyEIBpTqfoav93Vjkp49N-NoECW0rLLg-cJqzjEVfESz3oPQuBA-ab7xZC__LCeaDUL4TygdbnNV8J5SXMTcfH_TNGtQhNRqM-8txL8KgzwsrTThgWU7LnLKI3e8xiDK2BjwP0gzalfEgO66c-afIH7K1lOs</recordid><startdate>19980801</startdate><enddate>19980801</enddate><creator>Vicent, G.P.</creator><creator>Burton, G.</creator><creator>Ghini, A.</creator><creator>Lantos, C.P.</creator><creator>Galigniana, M.D.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980801</creationdate><title>Influence of calf serum on glucocorticoid-responses of certain progesterone derivatives</title><author>Vicent, G.P. ; Burton, G. ; Ghini, A. ; Lantos, C.P. ; Galigniana, M.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-ed65408f6f734217e8ec904efba6a7bade29dfff76abe97e1740efcabc4de77f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Adrenalectomy</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>Cattle</topic><topic>Cell physiology</topic><topic>Cells, Cultured</topic><topic>Corticosterone - metabolism</topic><topic>Cytosol - metabolism</topic><topic>Dexamethasone - pharmacology</topic><topic>Enzyme Induction</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormonal regulation</topic><topic>HSP90 Heat-Shock Proteins - metabolism</topic><topic>Liver - drug effects</topic><topic>Liver - enzymology</topic><topic>Liver - metabolism</topic><topic>Liver Glycogen - biosynthesis</topic><topic>Male</topic><topic>Methionine - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular and cellular biology</topic><topic>Progesterone - analogs & derivatives</topic><topic>Progesterone - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, Glucocorticoid - metabolism</topic><topic>Structure-Activity Relationship</topic><topic>Thymus Gland - drug effects</topic><topic>Thymus Gland - metabolism</topic><topic>Transcortin - metabolism</topic><topic>Tyrosine Transaminase - biosynthesis</topic><topic>Uridine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vicent, G.P.</creatorcontrib><creatorcontrib>Burton, G.</creatorcontrib><creatorcontrib>Ghini, A.</creatorcontrib><creatorcontrib>Lantos, C.P.</creatorcontrib><creatorcontrib>Galigniana, M.D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of steroid biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vicent, G.P.</au><au>Burton, G.</au><au>Ghini, A.</au><au>Lantos, C.P.</au><au>Galigniana, M.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of calf serum on glucocorticoid-responses of certain progesterone derivatives</atitle><jtitle>The Journal of steroid biochemistry and molecular biology</jtitle><addtitle>J Steroid Biochem Mol Biol</addtitle><date>1998-08-01</date><risdate>1998</risdate><volume>66</volume><issue>4</issue><spage>211</spage><epage>216</epage><pages>211-216</pages><issn>0960-0760</issn><eissn>1879-1220</eissn><abstract>The following
in vitro glucocorticoid (GC) parameters of progesterone (P), 1-ene progesterone (ΔP), 11
β-hydroxyprogesterone (HOP), 11
β-1-ene progesterone (ΔHOP) and dexamethasone (Dexa) were assayed in the presence or absence of bovine calf serum (BCS): binding to thymus cytosol, dissociation of the glucocorticoid receptor (GR)–heat shock protein 9O (hsp90) complex (diss.), tyrosine aminotransferase (TAT) induction in hepatocytes and the inhibition of
3H-uridine and
35S-methionine uptake by thymocytes. Without BCS, steroids were in most cases active in this general order: Dex>ΔHOP>HOP>ΔP>P. BCS abolished all activities in P and ΔP, but left them unaltered in all other steroids, except diss. in HOP, which diminished intermediately. Binding of P, ΔP, HOP and ΔHOP to GR and CBG paralleled their
in vivo activating effects on glycogen deposition. Conclusions: in this steroid series, BCS, but not CBG, inhibits GC responses of P and ΔP. 11-
β hydroxylation frees those molecules from the inhibitory effects of BCS.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>9744518</pmid><doi>10.1016/S0960-0760(98)00040-5</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0960-0760 |
| ispartof | The Journal of steroid biochemistry and molecular biology, 1998-08, Vol.66 (4), p.211-216 |
| issn | 0960-0760 1879-1220 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73929101 |
| source | Elsevier |
| subjects | Adrenalectomy Animals Biological and medical sciences Blood Cattle Cell physiology Cells, Cultured Corticosterone - metabolism Cytosol - metabolism Dexamethasone - pharmacology Enzyme Induction Fundamental and applied biological sciences. Psychology Hormonal regulation HSP90 Heat-Shock Proteins - metabolism Liver - drug effects Liver - enzymology Liver - metabolism Liver Glycogen - biosynthesis Male Methionine - metabolism Mice Mice, Inbred BALB C Molecular and cellular biology Progesterone - analogs & derivatives Progesterone - pharmacology Rats Rats, Sprague-Dawley Receptors, Glucocorticoid - metabolism Structure-Activity Relationship Thymus Gland - drug effects Thymus Gland - metabolism Transcortin - metabolism Tyrosine Transaminase - biosynthesis Uridine - metabolism |
| title | Influence of calf serum on glucocorticoid-responses of certain progesterone derivatives |
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