Changed expression of 9-O-acetyl GD3 (CDw60) in benign and atypical proliferative lesions and carcinomas of the human breast

• Published in: Histochemistry and cell biology 1998-09, Vol.110 (3), p.217-229
• Main Authors: Gocht, A, Rutter, G, Kniep, B
• Format: Article
• Language: English
• Subjects: Adult; Aged; Antigens, CD - analysis; Antigens, Differentiation, T-Lymphocyte - analysis; Benign; Breast - immunology; Breast - ultrastructure; Breast cancer; Breast carcinoma; Breast Diseases - immunology; Breast Diseases - pathology; Breast Neoplasms - immunology; Breast Neoplasms - ultrastructure; Cell surface; Cytoplasm; Cytoplasm - immunology; Cytoplasm - ultrastructure; Epithelial cells; Female; Fluorescent Antibody Technique; Frozen Sections; Gangliosides; Glycosphingolipids - analysis; Golgi Apparatus - immunology; Golgi Apparatus - ultrastructure; Golgi cells; Gynecomastia; Humans; Hyperplasia; Immunohistochemistry; Invasiveness; Labeling; Lesions; Male; Mammary gland; Microscopy, Immunoelectron; Middle Aged; Monoclonal antibodies; Paraffin; Polarity; Thin-layer chromatography; Tumor cell lines; Tumor Cells, Cultured; Tumors
• ISSN: 0948-6143; 1432-119X
• DOI: 10.1007/s004180050284

Expression of gangliosides is affected in various ways by malignant cell transformation. In the present study, we investigated the expression of CDw60, a constituent of O-acetylated disialogangliosides, in benign and atypical proliferative breast diseases, and preinvasive and invasive carcinomas by immunohistochemistry and thin-layer chromatography (TLC). In normal ducts, antibodies to CDw60 (mAb M-T21) reacted to membranes of the Golgi apparatus in the juxtaluminal cell compartment. A similar polarized distribution of Golgi cisterns in epithelial cells was observed in several benign lesions, i.e., fibroadenomas, intraductal papillomas, and gynecomastia. In contrast, blunt duct adenosis and duct hyperplasia exhibited an abnormal cytosolic and cell surface staining, whereas atypical duct hyperplasia showed randomly dispersed immunoreactive Golgi cisterns, indicating loss of epithelial polarity. In mammary carcinomas and in two breast carcinoma cell lines (MCF-7 and EFM-19) the neoplastic cells contained CDw60-immunolabelled Golgi complexes, which were distributed in a disorderly fashion throughout the cytoplasm, thus reflecting a loss of epithelial polarity. Additionally, only well differentiated ductal carcinomas in situ or invasive ductal carcinomas disclosed a strong cell surface labelling, which was absent in lower differentiated carcinomas of the same types. In all carcinomas, the intensity of CDw60 immunostaining decreased with progressing loss of differentiation (grade of dedifferentiation), as demonstrated by staining intensity in paraffin sections and by evaluation of the relative amounts of extracted 9-O-acetyl GD3 by TLC. Our results indicate that abnormal CDw60 expression is already detectable in benign proliferative breast lesions with different risk rates to develop into malignant lesions. Downregulation of CDw60 expression in poorly differentiated invasive carcinomas may be the consequence of loss of cell functions usually associated with poor prognosis.