Isolation of a Serogroup 1-Specific Antigen from Legionella pneumophila

Outer membrane material was extracted from a serogroup 1 strain of Legionella pneumophila using ethylenediaminetetraacetate. Ferritin-conjugated antiserum reacted only at the surface of the organism, as seen by electron microscopy. Outer membrane material was fractionated into five peaks by molecula...

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Bibliographic Details
Published in:The Journal of infectious diseases 1982-02, Vol.145 (2), p.224-233
Main Authors: Flesher, Alan R., Jennings, Harold J., Lugowski, Czeslaw, Kasper, Dennis L.
Format: Article
Language:English
Subjects:
Amino acids
Animals
Antigens
Antigens, Bacterial - isolation & purification
Antiserum
Bacteria
Chromatography, Gel
Enzyme-Linked Immunosorbent Assay
Gels
Legionella - immunology
Legionella - ultrastructure
Legionella pneumophila
Legionnaires disease
Lipids
Materials
Medical Microbiology
Microscopy, Electron
Rabbits
Serotyping
Sodium
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Summary:Outer membrane material was extracted from a serogroup 1 strain of Legionella pneumophila using ethylenediaminetetraacetate. Ferritin-conjugated antiserum reacted only at the surface of the organism, as seen by electron microscopy. Outer membrane material was fractionated into five peaks by molecular sieve chromatography using a gel that had been equilibrated in a buffer containing sodium deoxycholate. One resolved peak (∾4 x 10⁴ daltons) was highly active serologically. When rechromatographed in the absence of sodium deoxycholate, material from this peak reaggregated to ∼ 10⁶ daltons. The serologic activity of this antigen was restricted to L. pneumophila serogroup 1, although minor cross-reactions with strains of serogroups 2 and 4 were detected using an enzyme-linked immunosorbent assay. The antigen was
ISSN:0022-1899
DOI:10.1093/infdis/145.2.224