Comparison of detection methods for liquid chromatographic determination of 3-nitro- l-tyrosine

A liquid chromatographic method has been developed for the determination of 3-nitro- l-tyrosine. Different detection methods, including UV, oxidative and redox electrochemistry, and postcolumn photolysis followed by electrochemical detection, have been optimized and compared in terms of analysis tim...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 1998-08, Vol.713 (2), p.289-295
Main Authors: Liu, Huwei, Huang, Tiehua, Kissinger, Candice B, Kissinger, Peter T
Format: Article
Language:English
Subjects:
3-Nitro- l-tyrosine
Aminoacids, peptides. Hormones. Neuropeptides
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Chromatography, Liquid - methods
Electrochemistry
Fundamental and applied biological sciences. Psychology
Microdialysis
Oxidation-Reduction
Photolysis
Proteins
Rats
Reproducibility of Results
Spectrophotometry, Ultraviolet
Tyrosine - analogs & derivatives
Tyrosine - blood
Tyrosine - chemistry
Tyrosine - radiation effects
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Summary:A liquid chromatographic method has been developed for the determination of 3-nitro- l-tyrosine. Different detection methods, including UV, oxidative and redox electrochemistry, and postcolumn photolysis followed by electrochemical detection, have been optimized and compared in terms of analysis time, detection limit and dynamic range. It was demonstrated that liquid chromatography with postcolumn photolysis followed by electrochemical detection is the most effective method, with an analysis time of 5 min, detection limit of 0.01 pmol, and a linear dynamic range from 2 n M to 100 μ M.
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(98)00167-4