Molecular cloning and characterization of a highly conserved chicken cellular nucleic acid binding protein cDNA

A chicken cellular nucleic acid binding protein (cCNBP) cDNA was isolated from a chicken Con-A-stimulated immune cell library by differential screening. cCNBP is a Cys/Cys–His/Cys zinc finger DNA binding protein of unknown function. The chicken CNBP nucleotide and deduced amino acid sequence showed...

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Bibliographic Details
Published in:Gene 1998-09, Vol.218 (1), p.95-101
Main Authors: Ruble, Dawn M, Foster, Douglas N
Format: Article
Language:English
Subjects:
ACIDE NUCLEIQUE
ACIDOS NUCLEICOS
Animals
Cells, Cultured
CHICKENS
Chickens - genetics
CLONACION MOLECULAR
CLONAGE MOLECULAIRE
Cloning, Molecular
CODE GENETIQUE
CODIGO GENETICO
COMPLEJOS INMUNOLOGICOS
COMPLEXE IMMUN
Con A immune cell stimulation
Concanavalin A
Conserved Sequence
Cys/Cys–His/Cys zinc finger binding protein
Differential screening
DNA, Complementary
DNA-Binding Proteins - genetics
Gene Expression
GENETIC CODE
Humans
IMMUNE COMPLEXES
Mice
MOLECULAR CLONING
Molecular Sequence Data
Northern blot analysis
NUCLEIC ACIDS
NUCLEOTIDE SEQUENCE
POLLO
POULET
Rats
RNA, Messenger - metabolism
RNA-Binding Proteins
SECUENCIA NUCLEOTIDICA
Sequence Homology, Amino Acid
SEQUENCE NUCLEOTIDIQUE
Spleen - cytology
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Summary:A chicken cellular nucleic acid binding protein (cCNBP) cDNA was isolated from a chicken Con-A-stimulated immune cell library by differential screening. cCNBP is a Cys/Cys–His/Cys zinc finger DNA binding protein of unknown function. The chicken CNBP nucleotide and deduced amino acid sequence showed extraordinary sequence conservation (between 81–98% similarities) when compared to human, mouse and rat CNBP. The CNBP gene was shown to be a single copy and to cross-hybridize to human and mouse genomic DNA. A Northern blot analysis revealed cCNBP to be a constitutively expressed gene in a wide variety of tissues and to be differentially expressed in cultured chicken spleen and bursal cells after mitogen stimulation.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(98)00383-7