In vivo addition of telomeric repeats to foreign DNA generates extrachromosomal DNAs in the taxol-producing fungus Pestalotiopsis microspora

Transformation of the taxol-producing filamentous fungus Pestalotiopsis microspora with a plasmid containing the bacterial hygromycin resistance gene fused to Aspergillus regulatory sequences resulted in the in vivo formation of extrachromosomal DNAs with telomeric repeats in the majority of transfo...

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Bibliographic Details
Published in:Fungal genetics and biology 1998-08, Vol.24 (3), p.335-344
Main Authors: Long, D M, Smidansky, E D, Archer, A J, Strobel, G A
Format: Article
Language:English
Subjects:
Aspergillus nidulans - genetics
Aspergillus niger - genetics
Base Sequence
Drug Resistance, Microbial - genetics
Escherichia coli - genetics
Genes, Fungal
Genetic Techniques
Mitosporic Fungi - genetics
Mitosporic Fungi - metabolism
Paclitaxel - biosynthesis
Pestalotiopsis microspora
Phenotype
Plasmids
Repetitive Sequences, Nucleic Acid
Restriction Mapping
Telomere - genetics
Transfection
Transformation, Genetic
Trees - microbiology
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Summary:Transformation of the taxol-producing filamentous fungus Pestalotiopsis microspora with a plasmid containing the bacterial hygromycin resistance gene fused to Aspergillus regulatory sequences resulted in the in vivo formation of extrachromosomal DNAs with telomeric repeats in the majority of transformants. Repeats of the telomeric sequence 5'-TTAGGG-3' were appended to nontelomeric transforming DNA termini. No fungal sequences other than telomeric repeats were detected in extrachromosomal DNAs. Transformants contained three to six different sizes or conformational forms of extrachromosomal DNAs. The DNAs showed no change in size or internal structure during 6 months of growth with selection, but were lost after 20 days of growth without selection. Transformation of wild-type P. microspora with a PCR-amplified extrachromosomal DNA having terminal telomeric repeats produced up to 50-fold more transformants than the original transformation vector. The addition of telomeric repeats to foreign DNA is unusual among fungi and may have important adaptive or developmental implications.
ISSN:1087-1845
DOI:10.1006/fgbi.1998.1065