A Mathematical Model Describing Tannin–Protein Association

A quantitative model is derived for tannin–protein binding and protein precipitation in solution. This model is based on the assumption that precipitation occurs when the number of tannin molecules associated with one protein molecule attains a critical value. Precipitation occurs at this point beca...

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Bibliographic Details
Published in:Analytical biochemistry 1998-10, Vol.263 (1), p.46-50
Main Authors: Silber, Moris L., Davitt, Bruce B., Khairutdinov, Rafail F., Hurst, James K.
Format: Article
Language:English
Subjects:
Chemical Precipitation
Hydrolyzable Tannins - chemistry
Hydrolyzable Tannins - metabolism
Mathematics
Models, Chemical
Proteins - chemistry
Proteins - metabolism
Rosaniline Dyes - chemistry
Rosaniline Dyes - metabolism
Serum Albumin, Bovine - chemistry
Serum Albumin, Bovine - metabolism
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Summary:A quantitative model is derived for tannin–protein binding and protein precipitation in solution. This model is based on the assumption that precipitation occurs when the number of tannin molecules associated with one protein molecule attains a critical value. Precipitation occurs at this point because tannin crosslinking causes formation of large protein aggregates. Analytical expressions were derived for the dependence of protein precipitate yields upon the concentrations of the protein and tannin in solution. This expression fits reasonably well the experimentally observed bell-shaped dependencies of bovine serum albumin or gelatin precipitation upon total protein and tannin concentrations.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1998.2817