Analytical and clinical performance characteristics of Tandem-MP Ostase, a new immunoassay for serum bone alkaline phosphatase

The performance characteristics of the Tandem-MP Ostase assay, a new microplate immunoassay for bone-specific alkaline phosphatase (bone ALP; EC 3.1.3.1) in human sera, are described. Bone ALP is bound to streptavidin-coated microwells by a single biotinylated anti-bone ALP monoclonal antibody. Anti...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 1998-10, Vol.44 (10), p.2139-2147
Main Authors: Broyles, Dennis L, Nielsen, Randall G, Bussett, Elizabeth M, Lu, W. Douglas, Mizrahi, Isaac A, Nunnelly, Patricia A, Ngo, Tram A, Noell, Julia, Christenson, Robert H, Kress, Barry C
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Alkaline Phosphatase - blood
Biological and medical sciences
Bone and Bones - enzymology
Bone Diseases - blood
Bone Diseases - drug therapy
Bone Diseases - enzymology
Bone Neoplasms - blood
Bone Neoplasms - enzymology
Bone Neoplasms - secondary
Child
Child, Preschool
Diphosphonates - therapeutic use
Female
Humans
Immunoenzyme Techniques
Investigative techniques, diagnostic techniques (general aspects)
Isoenzymes - blood
Liver - enzymology
Male
Medical sciences
Middle Aged
Osteitis Deformans - blood
Osteitis Deformans - drug therapy
Osteitis Deformans - enzymology
Osteoarticular system. Muscles
Pamidronate
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Prostatic Neoplasms - blood
Prostatic Neoplasms - pathology
Radioimmunoassay
Reference Values
Regression Analysis
Sensitivity and Specificity
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Summary:The performance characteristics of the Tandem-MP Ostase assay, a new microplate immunoassay for bone-specific alkaline phosphatase (bone ALP; EC 3.1.3.1) in human sera, are described. Bone ALP is bound to streptavidin-coated microwells by a single biotinylated anti-bone ALP monoclonal antibody. Antigen is detected by the addition of p-nitrophenyl phosphate. The assay is performed at room temperature in
ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/44.10.2139