Molecular cloning and sequence analysis of a chicken cDNA encoding tyrosinase-related protein-2/DOPAchrome tautomerase

We have cloned and sequenced a chicken cDNA encoding an l-DOPAchrome tautomerase (DCT) from an embryonic melanocyte cDNA library. The chicken DCT gene encodes a deduced protein of 516 amino acids (aas) and shares 69.2% and 69.9% aa sequence identity with the deduced mouse and human DCT proteins, res...

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Bibliographic Details
Published in:Gene 1998-09, Vol.219 (1), p.45-53
Main Authors: April, Craig S., Jackson, Ian J., Kidson, Susan H.
Format: Article
Language:English
Subjects:
ADN
Amino Acid Sequence
Animals
Base Sequence
Chick Embryo
CHICKENS
Chickens - genetics
CLONACION MOLECULAR
CLONAGE MOLECULAIRE
Cloning, Molecular
Consensus Sequence
DCT
DNA
DNA, Complementary
Humans
Intramolecular Oxidoreductases - biosynthesis
Intramolecular Oxidoreductases - chemistry
Intramolecular Oxidoreductases - genetics
MELANINAS
MELANINE
MELANINS
Melanocyte
Mice
MOLECULAR CLONING
Molecular Sequence Data
Multigene Family
NUCLEOTIDE SEQUENCE
Pigment
Pigment Epithelium of Eye - enzymology
PIGMENTACION
PIGMENTATION
POLLO
POULET
Recombinant Proteins - biosynthesis
Recombinant Proteins - chemistry
SECUENCIA NUCLEOTIDICA
Sequence Alignment
Sequence Homology, Amino Acid
SEQUENCE NUCLEOTIDIQUE
Transcription, Genetic
TYRP2
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Description
Summary:We have cloned and sequenced a chicken cDNA encoding an l-DOPAchrome tautomerase (DCT) from an embryonic melanocyte cDNA library. The chicken DCT gene encodes a deduced protein of 516 amino acids (aas) and shares 69.2% and 69.9% aa sequence identity with the deduced mouse and human DCT proteins, respectively. Northern blot hybridisation analysis reveals a DCT transcript of 3.5 kb in RNA from the retinal pigment epithelium (RPE) of chick embryos. Genomic Southern blot hybridisation analysis suggests that the chicken DCT gene consists of several introns and spans between 15 and 30 kb of the chicken genome. This study completes the sequencing of all the members of the chicken tyrosinase-related protein gene family and provides evidence that this gene family is conserved between avians and mammals.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(98)00403-X