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Molecular cloning and sequence analysis of a chicken cDNA encoding tyrosinase-related protein-2/DOPAchrome tautomerase
We have cloned and sequenced a chicken cDNA encoding an l-DOPAchrome tautomerase (DCT) from an embryonic melanocyte cDNA library. The chicken DCT gene encodes a deduced protein of 516 amino acids (aas) and shares 69.2% and 69.9% aa sequence identity with the deduced mouse and human DCT proteins, res...
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| Published in: | Gene 1998-09, Vol.219 (1), p.45-53 |
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| container_title | Gene |
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| creator | April, Craig S. Jackson, Ian J. Kidson, Susan H. |
| description | We have cloned and sequenced a chicken cDNA encoding an
l-DOPAchrome tautomerase (DCT) from an embryonic melanocyte cDNA library. The chicken
DCT gene encodes a deduced protein of 516 amino acids (aas) and shares 69.2% and 69.9% aa sequence identity with the deduced mouse and human DCT proteins, respectively. Northern blot hybridisation analysis reveals a
DCT transcript of 3.5
kb in RNA from the retinal pigment epithelium (RPE) of chick embryos. Genomic Southern blot hybridisation analysis suggests that the chicken
DCT gene consists of several introns and spans between 15 and 30
kb of the chicken genome. This study completes the sequencing of all the members of the chicken tyrosinase-related protein gene family and provides evidence that this gene family is conserved between avians and mammals. |
| doi_str_mv | 10.1016/S0378-1119(98)00403-X |
| format | article |
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l-DOPAchrome tautomerase (DCT) from an embryonic melanocyte cDNA library. The chicken
DCT gene encodes a deduced protein of 516 amino acids (aas) and shares 69.2% and 69.9% aa sequence identity with the deduced mouse and human DCT proteins, respectively. Northern blot hybridisation analysis reveals a
DCT transcript of 3.5
kb in RNA from the retinal pigment epithelium (RPE) of chick embryos. Genomic Southern blot hybridisation analysis suggests that the chicken
DCT gene consists of several introns and spans between 15 and 30
kb of the chicken genome. This study completes the sequencing of all the members of the chicken tyrosinase-related protein gene family and provides evidence that this gene family is conserved between avians and mammals.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/S0378-1119(98)00403-X</identifier><identifier>PMID: 9756992</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>ADN ; Amino Acid Sequence ; Animals ; Base Sequence ; Chick Embryo ; CHICKENS ; Chickens - genetics ; CLONACION MOLECULAR ; CLONAGE MOLECULAIRE ; Cloning, Molecular ; Consensus Sequence ; DCT ; DNA ; DNA, Complementary ; Humans ; Intramolecular Oxidoreductases - biosynthesis ; Intramolecular Oxidoreductases - chemistry ; Intramolecular Oxidoreductases - genetics ; MELANINAS ; MELANINE ; MELANINS ; Melanocyte ; Mice ; MOLECULAR CLONING ; Molecular Sequence Data ; Multigene Family ; NUCLEOTIDE SEQUENCE ; Pigment ; Pigment Epithelium of Eye - enzymology ; PIGMENTACION ; PIGMENTATION ; POLLO ; POULET ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - chemistry ; SECUENCIA NUCLEOTIDICA ; Sequence Alignment ; Sequence Homology, Amino Acid ; SEQUENCE NUCLEOTIDIQUE ; Transcription, Genetic ; TYRP2</subject><ispartof>Gene, 1998-09, Vol.219 (1), p.45-53</ispartof><rights>1998 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-b29152fc6297c48a3fdf6d76e29fbe9cd5e2762335aa9c1930f4675b1b225c3c3</citedby><cites>FETCH-LOGICAL-c413t-b29152fc6297c48a3fdf6d76e29fbe9cd5e2762335aa9c1930f4675b1b225c3c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9756992$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>April, Craig S.</creatorcontrib><creatorcontrib>Jackson, Ian J.</creatorcontrib><creatorcontrib>Kidson, Susan H.</creatorcontrib><title>Molecular cloning and sequence analysis of a chicken cDNA encoding tyrosinase-related protein-2/DOPAchrome tautomerase</title><title>Gene</title><addtitle>Gene</addtitle><description>We have cloned and sequenced a chicken cDNA encoding an
l-DOPAchrome tautomerase (DCT) from an embryonic melanocyte cDNA library. The chicken
DCT gene encodes a deduced protein of 516 amino acids (aas) and shares 69.2% and 69.9% aa sequence identity with the deduced mouse and human DCT proteins, respectively. Northern blot hybridisation analysis reveals a
DCT transcript of 3.5
kb in RNA from the retinal pigment epithelium (RPE) of chick embryos. Genomic Southern blot hybridisation analysis suggests that the chicken
DCT gene consists of several introns and spans between 15 and 30
kb of the chicken genome. This study completes the sequencing of all the members of the chicken tyrosinase-related protein gene family and provides evidence that this gene family is conserved between avians and mammals.</description><subject>ADN</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Chick Embryo</subject><subject>CHICKENS</subject><subject>Chickens - genetics</subject><subject>CLONACION MOLECULAR</subject><subject>CLONAGE MOLECULAIRE</subject><subject>Cloning, Molecular</subject><subject>Consensus Sequence</subject><subject>DCT</subject><subject>DNA</subject><subject>DNA, Complementary</subject><subject>Humans</subject><subject>Intramolecular Oxidoreductases - biosynthesis</subject><subject>Intramolecular Oxidoreductases - chemistry</subject><subject>Intramolecular Oxidoreductases - genetics</subject><subject>MELANINAS</subject><subject>MELANINE</subject><subject>MELANINS</subject><subject>Melanocyte</subject><subject>Mice</subject><subject>MOLECULAR CLONING</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>Pigment</subject><subject>Pigment Epithelium of Eye - enzymology</subject><subject>PIGMENTACION</subject><subject>PIGMENTATION</subject><subject>POLLO</subject><subject>POULET</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Recombinant Proteins - chemistry</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><subject>Transcription, Genetic</subject><subject>TYRP2</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqFkU9v1DAQxS0EKkvLR6jkE4JDqP_EcXxCqxYK0tJWAqTeLGc8aQ3ZuLWTSvvt6-2uyrG-jKz3xp55P0KOOfvMGW9OfjGp24pzbj6a9hNjNZPV9Suy4K02FWOyfU0Wz5a35F3Of1k5SokDcmC0aowRC_LwMw4I8-AShSGOYbyhbvQ04_2MI2C5uGGTQ6axp47CbYB_OFI4u1jSoke_bZg2KeYwuoxVwsFN6OldihOGsRInZ5dXS7hNcY10cvNUairGI_Kmd0PG9_t6SP58-_r79Hu1ujz_cbpcVVBzOVWdMFyJHhphNNStk73vG68bFKbv0IBXKHQjpFTOGeBGsr5utOp4J4QCCfKQfNi9WwYqG-XJrkMGHAY3Ypyz1dJozbh80ch1CZ23dTGqnRHK0jlhb-9SWLu0sZzZLRj7BMZuU7emtU9g7HXpO95_MHdr9M9dexL_9d5F625SyPZixY0xhVnd8qJ_2elY4noImGyGsEXkQ0KYrI_hhQkeAR61p10</recordid><startdate>19980928</startdate><enddate>19980928</enddate><creator>April, Craig S.</creator><creator>Jackson, Ian J.</creator><creator>Kidson, Susan H.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19980928</creationdate><title>Molecular cloning and sequence analysis of a chicken cDNA encoding tyrosinase-related protein-2/DOPAchrome tautomerase</title><author>April, Craig S. ; Jackson, Ian J. ; Kidson, Susan H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-b29152fc6297c48a3fdf6d76e29fbe9cd5e2762335aa9c1930f4675b1b225c3c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>ADN</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Chick Embryo</topic><topic>CHICKENS</topic><topic>Chickens - genetics</topic><topic>CLONACION MOLECULAR</topic><topic>CLONAGE MOLECULAIRE</topic><topic>Cloning, Molecular</topic><topic>Consensus Sequence</topic><topic>DCT</topic><topic>DNA</topic><topic>DNA, Complementary</topic><topic>Humans</topic><topic>Intramolecular Oxidoreductases - biosynthesis</topic><topic>Intramolecular Oxidoreductases - chemistry</topic><topic>Intramolecular Oxidoreductases - genetics</topic><topic>MELANINAS</topic><topic>MELANINE</topic><topic>MELANINS</topic><topic>Melanocyte</topic><topic>Mice</topic><topic>MOLECULAR CLONING</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>NUCLEOTIDE SEQUENCE</topic><topic>Pigment</topic><topic>Pigment Epithelium of Eye - enzymology</topic><topic>PIGMENTACION</topic><topic>PIGMENTATION</topic><topic>POLLO</topic><topic>POULET</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Recombinant Proteins - chemistry</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><topic>Transcription, Genetic</topic><topic>TYRP2</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>April, Craig S.</creatorcontrib><creatorcontrib>Jackson, Ian J.</creatorcontrib><creatorcontrib>Kidson, Susan H.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>April, Craig S.</au><au>Jackson, Ian J.</au><au>Kidson, Susan H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and sequence analysis of a chicken cDNA encoding tyrosinase-related protein-2/DOPAchrome tautomerase</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1998-09-28</date><risdate>1998</risdate><volume>219</volume><issue>1</issue><spage>45</spage><epage>53</epage><pages>45-53</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>We have cloned and sequenced a chicken cDNA encoding an
l-DOPAchrome tautomerase (DCT) from an embryonic melanocyte cDNA library. The chicken
DCT gene encodes a deduced protein of 516 amino acids (aas) and shares 69.2% and 69.9% aa sequence identity with the deduced mouse and human DCT proteins, respectively. Northern blot hybridisation analysis reveals a
DCT transcript of 3.5
kb in RNA from the retinal pigment epithelium (RPE) of chick embryos. Genomic Southern blot hybridisation analysis suggests that the chicken
DCT gene consists of several introns and spans between 15 and 30
kb of the chicken genome. This study completes the sequencing of all the members of the chicken tyrosinase-related protein gene family and provides evidence that this gene family is conserved between avians and mammals.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9756992</pmid><doi>10.1016/S0378-1119(98)00403-X</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Gene, 1998-09, Vol.219 (1), p.45-53 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73977013 |
| source | Elsevier |
| subjects | ADN Amino Acid Sequence Animals Base Sequence Chick Embryo CHICKENS Chickens - genetics CLONACION MOLECULAR CLONAGE MOLECULAIRE Cloning, Molecular Consensus Sequence DCT DNA DNA, Complementary Humans Intramolecular Oxidoreductases - biosynthesis Intramolecular Oxidoreductases - chemistry Intramolecular Oxidoreductases - genetics MELANINAS MELANINE MELANINS Melanocyte Mice MOLECULAR CLONING Molecular Sequence Data Multigene Family NUCLEOTIDE SEQUENCE Pigment Pigment Epithelium of Eye - enzymology PIGMENTACION PIGMENTATION POLLO POULET Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry SECUENCIA NUCLEOTIDICA Sequence Alignment Sequence Homology, Amino Acid SEQUENCE NUCLEOTIDIQUE Transcription, Genetic TYRP2 |
| title | Molecular cloning and sequence analysis of a chicken cDNA encoding tyrosinase-related protein-2/DOPAchrome tautomerase |
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