Histone proteins in HeLa S3 cells are synthesized in a cell cycle stage specific manner

The synthesis of histone proteins in G1 and S phase HeLa S3 cells was examined by two-dimensional electrophoretic fractionation of nuclear and total cellular proteins. Newly synthesized histones were detected only in S phase cells. Histone messenger RNA sequences, as detected by hybridization with c...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1982-02, Vol.215 (4533), p.683-685
Main Authors: Marashi, F, Baumbach, L, Rickles, R, Sierra, F, Stein, J L, Stein, G S
Format: Article
Language:English
Subjects:
Cell Cycle
Cell Nucleus - metabolism
Cytarabine - pharmacology
Cytoplasm - metabolism
Female
HeLa Cells - metabolism
Histones - biosynthesis
Humans
Protein Biosynthesis
Transcription, Genetic
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The synthesis of histone proteins in G1 and S phase HeLa S3 cells was examined by two-dimensional electrophoretic fractionation of nuclear and total cellular proteins. Newly synthesized histones were detected only in S phase cells. Histone messenger RNA sequences, as detected by hybridization with cloned human histone genes, were present in the cytoplasm of S phase but not G1 cells.
ISSN:0036-8075
DOI:10.1126/science.7058333